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Study on the Effect and Mechanism of miR-185 on Lower Extremity Deep Venous Thrombosis

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Abstract

Lower extremity deep venous thrombosis (LEDVT) is a venous reflux disorder caused by abnormal coagulation of blood. LEDVT can obstruct the lumen and LEDVT is the third vascular disease after cerebrovascular diseases and coronary artery diseases. miRNAs are associated with thrombosis, and miR-185 was reported to affect the proliferation and apoptosis of vascular endothelial cells by regulating receptor of advanced glycation end products (RAGE). However, no study has reported the effect of miR-185 on LEDVT. Here, we studied the effects of miR-185 on the PI3K/AKT and MAPK signaling pathways in the LEDVT cells. The results showed that miR-185 promotes cell proliferation through activating the PI3K/AKT and MAPK signaling pathways and then inhibits tissue factor and fibrin expression to reduce thrombosis. In short, our study provides new ideas and a theoretical basis for research on the prevention, diagnosis, and treatment of LEDVT.

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Data Availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Natural Science Research Projects Fund in Colleges and Universities of Anhui Province (Grant No. KJ2019A0327).

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Authors and Affiliations

  1. Department of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road, Bengbu, 233004, Anhui, People’s Republic of China

    Ran Lu, Wei Zhu, Haobo Sun, Liang Zhang, Zhaowen Yu, Zhonglin Nie & Yong Gao

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  1. Ran Lu
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  2. Wei Zhu
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  3. Haobo Sun
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  4. Liang Zhang
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  5. Zhaowen Yu
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  6. Zhonglin Nie
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  7. Yong Gao
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Corresponding author

Correspondence to Yong Gao.

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The authors declare that they have no conflict of interest.

Ethical Approval

All experimental protocols were conducted in accordance with the principles and procedures outlined in the “Guide for Care and Use of Laboratory animals” and approved by the Ethics Committee of the First Affiliated Hospital of Bengbu Medical College.

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Lu, R., Zhu, W., Sun, H. et al. Study on the Effect and Mechanism of miR-185 on Lower Extremity Deep Venous Thrombosis. Mol Biotechnol 64, 330–337 (2022). https://doi.org/10.1007/s12033-021-00412-w

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  • DOI: https://doi.org/10.1007/s12033-021-00412-w

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