Ectopic Expression of Rice PYL3 Enhances Cold and Drought Tolerance in Arabidopsis thaliana
Abscisic acid (ABA) plays an important role in plant development and adaptation to abiotic stresses. The pyrabactin resistance-like (PYL) gene family has been characterized as intracellular ABA receptors in Arabidopsis. We describe here the functional characterization of PYL3 ABA receptor from a drought-tolerant rice landrace Nagina 22 (N22). The induced expression level of the PYL3 transcript was observed in the N22 under different stress treatments, including cold, drought, high temperature, salt and ABA. In contrast, the expression of PYL3 was down-regulated in drought-susceptible rice cv. IR64 in response to above stresses. C-terminal GFP translational fusion of OsPYL3 was localized to both cytosol and nucleus explaining in part functional conservation of PYL protein as ABA receptor. Arabidopsis transgenic lines overexpressing OsPYL3 were hypersensitive to ABA suggesting ABA signaling pathway-dependent molecular response of the OsPYL3. Further, constitutive overexpression of OsPYL3 in Arabidopsis led to improved cold and drought stress tolerance. Thus, OsPYL3 identified in this study could be a good candidate for genetic improvement of cold and drought stress tolerance of rice and other crop plants.
KeywordsABA receptor Rice Cold Drought PYL Abiotic stress
This work was supported by the Indian Council of Agricultural Research (ICAR)-sponsored Network Project on Transgenics in Crops (NPTC). SKL acknowledges the University Grants Commission (UGC) and the Council of Scientific and Industrial Research (CSIR) for a CSIR-UGC JRF and SRF fellowship. SKM acknowledges the Department of Science and Technology (DST) for a DST-INSPIRE fellowship. The plants were grown in a space provided by the National Phytotron Facility, IARI. VC was supported by NASF (ICAR) project (Grant No. NFBSFARA/Phen 2015). Assistance provided by Mr. Amit K. Singh and Mr. Jeet B. Singh for growing rice and Arabidopsis plants is acknowledged.
SKL did all the experiments, experimental design and drafted the manuscript. SKM performed ABA and cold phenotyping of the transgenic Arabidopsis on plates. VC and KCB participated in experimental design and manuscript writing. All authors read and approved the final manuscript.
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