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Chimeric Virus as a Source of the Potato Leafroll Virus Antigen

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Abstract

Large quantities of potato leafroll virus (PLRV) antigen are difficult to obtain because this virus accumulates in plants at a low titer. To overcome this problem, we constructed a binary vector containing chimeric cDNA, in which the coat protein (CP) gene of the crucifer infecting tobacco mosaic virus (crTMV) was substituted for the coat protein gene of PLRV. The PLRV movement protein (MP) gene, which overlaps completely with the CP gene, was doubly mutated to eliminate priming of the PLRV MP translation from ATG codons with no changes to the amino acid sequence of the CP. The untranslated long intergenic region located upstream of the CP gene was removed from the construct. Transcribed powerful tobamovirus polymerase of the produced vector synthesized PLRV CP gene that was, in turn, translated into the protein. CP PLRV packed RNAs from the helical crTMV in spherical virions. Morphology, size and antigenic specificities of the wild-type and chimeric virus were similar. The yield of isolated chimera was about three orders higher than the yield of native PLRV. The genetic manipulations facilitated the generation of antibodies against the chimeric virus, which recognize the wild-type PLRV.

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References

  1. Mayo, M. A., Barker, H. D., Robinson, J., Tamada, T., & Harrison, B. D. (1982). Evidence that potato leafroll virus RNA is positive-stranded, is linked to a small protein and does not contain polyadenylate. Journal of General Virology, 59, 163–167.

    Article  CAS  Google Scholar 

  2. Taliansky, M., Mayo, M. A., & Barker, H. (2003). Potato leafroll virus: A classic pathogen shows some new tricks. Molecular Plant Pathology, 4, 81–89.

    Article  CAS  Google Scholar 

  3. van der Wilk, F., Huisman, M. J., Cornelisse, B. J., Huttinga, H., & Goldbach, R. (1989). Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Letters, 245, 51–56.

    Article  Google Scholar 

  4. van der Wilk, F., Verbeek, M., Dullemans, A. M., & van den Heuvel, J. F. (1997). The genome linked protein of potato leafroll virus is located downstream of the putative protease domain of the ORF1 product. Virology, 234, 300–303.

    Article  Google Scholar 

  5. Zhuo, T., Li, Y.-Y., Xiang, H.-Y., Wu, Z.-Y., Wang, X.-B., Wang, Y., et al. (2014). Amino acid sequence motifs essential for P0-mediated suppression of RNA silencing in an isolate of potato leafroll virus from inner Mongolia. Molecular Plant–Microbe Interactions, 27, 515–527.

    Article  CAS  Google Scholar 

  6. Smirnova, E., Firth, A. E., Miller, W. A., Scheidecker, D., Brault, V., Reinbold, C., et al. (2015). Discovery of a small non-AUG-initiated ORF in poleroviruses and luteoviruses that is required for long-distance movement. PLoS Pathogens, 11(5), e1004868.

    Article  Google Scholar 

  7. Mayo, M. A., & Ziegler-Graff, V. (1996). Molecular biology of luteoviruses. Advances in Virus Research, 46, 413–460.

    Article  CAS  Google Scholar 

  8. Syller, J. (1996). Potato leafroll virus (PLRV): Its transmission and control. Integrated Pest Management Reviews, 1, 217–227.

    Article  Google Scholar 

  9. Taliansky, M. E., & Robinson, D. J. (2003). Molecular biology of umbraviruses: Phantom warriors. Journal of General Virology, 84, 1951–1960.

    Article  CAS  Google Scholar 

  10. Lee, L., Palukaitis, P., & Gray, S. M. (2002). Host-dependent requirement for the potato leafroll virus 17-kDa protein in virus movement. Molecular Plant–Microbe Interactions, 15, 1086–1094.

    Article  CAS  Google Scholar 

  11. Rowhani, A., & Stace-Smith, R. (1979). Purification and characterization of potato leafroll virus. Virology, 98, 45–54.

    Article  CAS  Google Scholar 

  12. Takanami, Y., & Kubo, S. (1979). Enzyme-assisted purification of two phloem-limited plant viruses: Tobacco necrotic dwarf and potato leafroll. Journal of General Virology, 44, 153–159.

    Article  Google Scholar 

  13. Dorokhov, Y. L., Ivanov, P. A., Novikov, V. K., Agranovsky, A. A., Morozov, S. Y., Efimov, V. A., et al. (1994). Complete nucleotide sequence and genome organization of a tobamovirus infecting cruciferae plants. FEBS Letters, 350, 5–8.

    Article  CAS  Google Scholar 

  14. Sambrook, J., Fritsch, E. F., & Maniatis, T. (1987). Molecular cloning: A laboratory manual (2nd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  15. Voinnet, O. O., Pinto, Y. M., & Baulcombe, D. C. (1999). Suppression of gene silencing: A general strategy used by diverse DNA and RNA viruses of plants. Proceedings of the National Academy of Sciences, 96, 14147–14152.

    Article  CAS  Google Scholar 

  16. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  17. Nikitin, N., Trifonova, E., Karpova, O., & Atabekov, J. (2013). Examination of biologically active nanocomplexes by nanoparticle tracking analysis. Microscopy and Microanalysis, 19, 808–813.

    Article  CAS  Google Scholar 

  18. Fraenkel-Conrat, H., Singer, B., & Tsugita, A. (1961). Purification of viral RNA by means of bentonite. Virology, 14, 54–58.

    Article  CAS  Google Scholar 

  19. Chomczynski, P., & Mackey, K. (1995). Modification of the TRIZOL reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. BioTechniques, 19, 942–945.

    CAS  Google Scholar 

  20. Drygin, Y. F., Afonina, I. A., Bayer, K., Nikolaeva, O. V., & Atabekov, J. G. (1989). Diagnostics of X and M potato virus infections in crude tuber extracts by non-radioactive DNA-probing. Bioorganicheskaya Khimiya, 15, 947–951. (in Russian).

    CAS  Google Scholar 

  21. Harlow, E., & Lane, D. (1988). Antibodies. Cold Spring Harbor Laboratory, NY: A laboratory manual.

    Google Scholar 

  22. Clark, M. F., & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475–483.

    Article  CAS  Google Scholar 

  23. Jeevalatha, A., Kaundal, P., Shandil, R. K., Sharma, N. N., Chakrabarti, S. K., & Singh, B. P. (2013). Complete genome sequence of potato leafroll virus isolates infecting potato in the different geographical areas of India shows low level genetic diversity. Indian Journal of Virology, 24, 199–204.

    Article  CAS  Google Scholar 

  24. Huehnlein, A., Schubert, J., Thieme, T., Zahn, V., & Steinbach, P. (2012) Potato leafroll virus isolate SymlessLS10, complete genome GenBank 346189.1.

  25. Franco-Lara, L. F., McGeachy, K. D., Commandeur, U., Martin, R. R., Mayo, M. A., & Barker, H. (1999). Transformation of tobacco and potato with DNA encoding the full-length genome of potato leafroll virus: evidence for a novel virus distribution and host effects on virus multiplication. Journal of General Virology, 80, 2813–2822.

    Article  CAS  Google Scholar 

  26. Dorokhov, Y. L., Skurat, E. V., Frolova, O. Y., Gasanova, T. V., Smirnov, A. A., Zvereva, S. D., et al. (2004). Reciprocal dependence between pectinmethylesterase gene expression and tobamovirus reproduction effectiveness in Nicotiana benthamiana. Doklady Biochemistry and Biophysics, 394, 30–42.

    Article  CAS  Google Scholar 

  27. Kondakova, O. A., Butenko, K. O., Skurat, E. V., & Drygin, Y. F. (2016). Diagnostics of potato infections with PVY and PLRV by immunochromatography. Moscow University Biological Sciences Bulletin, 71, 39–44.

    Article  Google Scholar 

  28. Roberts, I. M., Tamada, T., & Harrison, B. D. (1980). Relationship of potato leafroll virus to luteoviruses: evidence from electron microscope serological tests. Journal of General Virology, 47, 209–213.

    Article  Google Scholar 

  29. Bonhoeffer, F., & Schachman, H. K. (1960). Studies on the organization of nucleic acids within nucleoproteins. Biochemical and Biophysical Research Communications, 2, 366–371.

    Article  Google Scholar 

  30. Gibbs, A. J. (1977). Descriptions of plant viruses. Canberra, Australia: Research School of Biological Sciences, Australian National University.

    Google Scholar 

  31. Fraenkel-Conrat, H., & Williams, R. C. (1955). Reconstitution of active tobacco mosaic virus from its inactive protein and nucleic acid components. Proceedings of the National Academy of Sciences, 41, 690–698.

    Article  CAS  Google Scholar 

  32. Atabekov, J. G., Novikov, V. K., Vishnichenko, V. K., & Kaftanova, A. S. (1970). Some properties of hybrid viruses reassembled in vitro. Virology, 41, 519–532.

    Article  CAS  Google Scholar 

  33. Dodds, J. A., & Hamilton, R. I. (1974). Masking of the RNA genome of tobacco mosaic virus by the protein of barley stripe mosaic virus in doubly infected barley. Virology, 59, 418–427.

    CAS  Google Scholar 

  34. Cañizares, M. C., Nicholson, L., & Lomonossoff, G. P. (2005). Use of viral vectors for vaccine production in plants. Immunology and Cell Biology, 83, 263–270.

    Article  Google Scholar 

  35. Rao, A. L. (2006). Genome packaging by spherical plant RNA viruses. Annual review of Phytopathology, 44, 61–87.

    Article  CAS  Google Scholar 

  36. Spitsin, S., Steplewski, K., Fleysh, N., Belanger, H., Mikheeva, T., Shivprasad, S., et al. (1999). Expression of alfalfa mosaic virus coat protein in tobacco mosaic virus (TMV) deficient in the production of its native coat protein supports long-distance movement of a chimeric TMV. Proceedings of the National Academy of Sciences of the United States of America, 96, 2549–2553.

    Article  CAS  Google Scholar 

  37. Waterhause, P. M., & Murant, A. F. (1983). Further evidence of the nature of the dependence of carrot mottle virus on carrot red leaf virus for transmission by aphids. Annals of Applied Biology, 103, 455–464.

    Article  Google Scholar 

  38. Barker, H., McGeachy, K. D., Ryabov, T. G., Commandeur, U., Mayo, V. F., & Taliansky, M. (2001). Evidence for RNA-mediated defense effects on the accumulation of potato leafroll virus. Journal of General Virology, 82, 3099–3106.

    Article  CAS  Google Scholar 

  39. Carrington, J. C., Kasschau, K. D., Mahajan, S. K., & Schaad, M. C. (1996). Cell-to-cell and long-distance transport of viruses in plants. Plant Cell, 8, 1669–1681.

    Article  CAS  Google Scholar 

  40. Citovsky, V., & Zambryski, P. (1993). Transport of nucleic acids through membrane channels-snaking through small holes. Annual Review of Microbiology, 47, 167–197.

    Article  CAS  Google Scholar 

  41. Tacke, E., Prufer, D., Shcmitz, J., & Rohde, W. (1991). The potato leafroll luteovirus 17 K protein is a single-stranded nucleic acid binding protein. Journal of General Virology, 72, 2035–2038.

    Article  CAS  Google Scholar 

  42. Taliansky, M., & Barker, H. (1999). Movement of luteoviruses in infected plants. In H. G. Smith & H. Barker (Eds.), The Luteoviridae (pp. 69–81). Wallingford: CAB International.

    Google Scholar 

  43. Luna, E., Pastor, V., Robert, J., Flors, V., Mauch-Mani, B., & Ton, J. (2011). Callose deposition: A multifaceted plant defense response. Molecular Plant–Microbe Interactions, 24, 183–193.

    Article  CAS  Google Scholar 

  44. Loniewska-Lwowska, A., Chełstowska, S., Zagórski-Ostoja, W., & Pałucha, A. E. (2009). Elements regulating potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain. Acta Biochimica Polonica, 56, 619–625.

    CAS  Google Scholar 

  45. Levy, A., Zheng, J. Y., & Lazarowitz, S. G. (2013). The tobamovirus turnip vein clearing virus 30-kilodalton movement protein localizes to novel nuclear filaments to enhance virus infection. Journal of Virology, 87, 6428–6440.

    Article  CAS  Google Scholar 

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Acknowledgments

We deeply appreciate Drs. A. Agranovsky, V. Hallan and E. Gavryushina for their critical comments and helpful discussion. We are thankful to Dr. Yu. A. Varitzev for the wild-type isolate of PLRV and gift of antibodies against PLRV from Agdia (USA).

Funding

This study was funded by the Russian Science Foundation (Grant No. 14-24-00007).

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Authors and Affiliations

  1. Department of Biology, Lomonosov Moscow State University, Moscow, Russian Federation, 119992

    Eugene V. Skurat, Konstantin O. Butenko, Olga A. Kondakova, Nikolai A. Nikitin, Olga V. Karpova & Joseph G. Atabekov

  2. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation, 119992

    Yuri F. Drygin & Joseph G. Atabekov

Authors
  1. Eugene V. Skurat
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  2. Konstantin O. Butenko
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  4. Nikolai A. Nikitin
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  5. Olga V. Karpova
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  6. Yuri F. Drygin
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  7. Joseph G. Atabekov
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Corresponding author

Correspondence to Yuri F. Drygin.

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All authors declare no conflict of interest.

Ethical approval

All experiments on animals (rabbits) were carried out in accordance with the animal care regulations of the M.V. Lomonosov Moscow State University. The protocol was approved by the Bioethics Committee of the Faculty of Biology, M.V. Lomonosov Moscow State University.

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Research involving Human Participants Informed consent: not applicable for this study.

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Informed consent was obtained from all individual participants included in the study.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Skurat, E.V., Butenko, K.O., Kondakova, O.A. et al. Chimeric Virus as a Source of the Potato Leafroll Virus Antigen. Mol Biotechnol 59, 469–481 (2017). https://doi.org/10.1007/s12033-017-0035-6

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  • DOI: https://doi.org/10.1007/s12033-017-0035-6

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