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Tobacco Rattle Virus-Based Silencing of Enoyl-CoA Reductase Gene and Its Role in Resistance Against Cotton Wilt Disease

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Abstract

A Tobacco rattle virus (TRV)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (Gossypium hirsutum). This approach was used to investigate the function of the Enoyl-CoA reductase (GhECR) gene in pathogen defense. Amino acid sequence alignment of Arabidopsis ECR with homologous sequence from G. hirsutum, G. arboreum, G. herbaceum and G. barbadense showed that ECRs are highly conserved among these species. TRV-based silencing of GhECR gene in G. hirsutum induced a cell death/necrotic lesion-like phenotype. Reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative PCR showed reduced GhECR mRNA levels in TRV inoculated plants. Three isolates of Verticillium dahliae (V. dahliae) and Fusarium oxysporum f. sp. vasinfectum (FOV) were used to infect GhECR-silenced plants. Out of 6 races of 2 pathogens, down regulation of GhECR gene resulted in reduced resistance. This is the first report showing that cotton GhECR gene is involved in resistance to different strains of V. dahliae and FOV.

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Acknowledgements

The authors are grateful to Dr. Ping He (Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology) and Dr. Libo Shan (Department of Plant Pathology and Microbiology) Texas A&M University for providing strains for fungal infections. A part of study is based upon work supported by Higher Education Commission (HEC) Govt. of Pakistan under the International Research Support Initiative Program (IRSIP). The study is in part supported by the “Pak-US cotton productivity enhancement program” of the International Center for Agricultural Research in the Dry Areas (ICARDA) funded by United States Department of Agriculture (USDA), Agricultural Research Service (ARS), under Agreement No. 58-6402-0-178F. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA, ICARDA or HEC. The authors thank Dr. Rob Briddon for critical reading of the manuscript and suggestions for the improvements. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan

    Roma Mustafa, Muhammad Hamza, Hira Kamal, Shahid Mansoor & Imran Amin

  2. Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan

    Roma Mustafa, Muhammad Hamza & Hira Kamal

  3. Crop Genetics Research Unit, USDA, Stoneville, MS, 38776, USA

    Jodi Scheffler

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  1. Roma Mustafa
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  2. Muhammad Hamza
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  3. Hira Kamal
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  4. Shahid Mansoor
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Contributions

RM, MH and HK did experimental work and RM wrote the first draft. SM and IA coordinated the research work. JS provided scientific and technical support. SM, IA and JS edited the paper. All authors have read and approved the manuscript.

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Correspondence to Imran Amin.

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Mustafa, R., Hamza, M., Kamal, H. et al. Tobacco Rattle Virus-Based Silencing of Enoyl-CoA Reductase Gene and Its Role in Resistance Against Cotton Wilt Disease. Mol Biotechnol 59, 241–250 (2017). https://doi.org/10.1007/s12033-017-0014-y

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