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In Vitro Encapsulation of Heterologous dsDNA Into Human Parvovirus B19 Virus-Like Particles

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Abstract

Virus-like particles (VLPs) have vast potential for applications in nanoscience and nanomedicine. These biological nanoparticles may be used for medical imaging, vaccination, or tissue-specific delivery of drugs or other bioactive molecules. VLPs of Human parvovirus B19 (B19 V) can be assembled in vitro from the recombinant VP2 protein. In this research, we describe a simple method for the encapsulation of heterologous linear dsDNA fragments of different sizes into B19 V-VP2 VLPs, in which the DNA and denatured VP2 protein are co-incubated and the assembly process is conducted by one dialysis step. Characterization of the particles by qPCR demonstrated the encapsulation of dsDNA, and indicates that the length of the dsDNA is critical for the encapsulation process. The strategy presented here opens the possibility to use this VLPs as a delivery system with future therapeutically applications.

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Acknowledgments

This work was funded by Consejo Nacional de Ciencia y Tecnología (CONACyT), México (Grant CB-2010-01-151189) and Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT), UNAM (Grant IN215613). The authors acknowledge technical support of Laura Álvarez-Añorve.

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  1. Sandra Paola Sánchez-Rodríguez

    Present address: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, 04510, Mexico

    Sandra Paola Sánchez-Rodríguez, Joana Valeria Enrriquez-Avila, Juan Miguel Soto-Fajardo & Ismael Bustos-Jaimes

  2. Department of Food Science and Biotechnology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico

    Carolina Peña-Montes

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  1. Sandra Paola Sánchez-Rodríguez
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Correspondence to Ismael Bustos-Jaimes.

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Sánchez-Rodríguez, S.P., Enrriquez-Avila, J.V., Soto-Fajardo, J.M. et al. In Vitro Encapsulation of Heterologous dsDNA Into Human Parvovirus B19 Virus-Like Particles. Mol Biotechnol 57, 309–317 (2015). https://doi.org/10.1007/s12033-014-9823-4

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  • DOI: https://doi.org/10.1007/s12033-014-9823-4

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