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Oligoprogenitor Cells Derived from Spermatogonia Stem Cells Improve Remyelination in Demyelination Model

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Abstract

Embryonic stem (ES) like cells-derived testis represents a possible alternative to replace of neurons and glia. Here, we differentiated spermatogonia cells to oligoprogenitor (OP) like cells and transplanted them to demyelination model and assess their recovery potential in a demyelinated corpus callosum model in rats. ES like cells were differentiated to OP like cells using appropriate inducers and were transplanted in situ to demyelinated corpus callosum. Cell integration as well as demyelination extension and myelination intensity changes were evaluated using histologic studies and immunocytochemistry after 2 and 4 weeks post transplantation. Investigation of Nestin, NF68, Olig2, and NG2 by immunocytochemical technique indicated the differentiation of ES like cells to neuroprogenitor and oligodendrocyte like cells in each induction stage. Histologic findings showed a significant decrease in demyelination extension and a significant increase in remyelination intensity in cell transplanted groups. Also on the base of PLP expression, differentiation of transplanted cells was confirmed to myelinogenic cells using immunocytochemistry technique. We conclude that ES like cells derived from spermatogonia cells can be differentiated to OP like cells that can form myelin after transplantation into the demyelination model in rat, this represents recovery potential of spermatogonia cells which opens new window for cell therapeutic approaches using spermatogonial stem cells.

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Acknowledgments

This research is derived from PhD thesis of Anatomical science with funding support from Medical Faculty of Tarbiat Modares University and Iranian council of stem cell technology.

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Authors and Affiliations

  1. Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Jalale-Ale-Ahmad Highway, P.O. Box 14115-175, Tehran, Iran

    M. Nazm Bojnordi, M. Movahedin & T. Tiraihi

  2. Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, P.O. Box 48471-91971, Sari, Iran

    M. Nazm Bojnordi & H. Ghasemi Hamidabadi

  3. Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Jalale-Ale-Ahmad Highway, P.O. Box 14115-175, Tehran, Iran

    M. Javan

Authors
  1. M. Nazm Bojnordi
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  2. M. Movahedin
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  3. T. Tiraihi
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  4. M. Javan
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  5. H. Ghasemi Hamidabadi
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Correspondence to M. Nazm Bojnordi or M. Movahedin.

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Nazm Bojnordi, M., Movahedin, M., Tiraihi, T. et al. Oligoprogenitor Cells Derived from Spermatogonia Stem Cells Improve Remyelination in Demyelination Model. Mol Biotechnol 56, 387–393 (2014). https://doi.org/10.1007/s12033-013-9722-0

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