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Establishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells

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Abstract

The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

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Acknowledgments

The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.

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Authors and Affiliations

  1. Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki Higashi-ku, Fukuoka, 812-8581, Japan

    Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Takahiro Kusakabe & Jae Man Lee

  2. Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki Higashi-ku, Fukuoka, 812-8581, Japan

    Kazuhiro Iiyama

Authors
  1. Jian Xu
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  2. Kaito Yoshimura
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  3. Hiroaki Mon
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  4. Zhiqing Li
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  5. Li Zhu
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  6. Kazuhiro Iiyama
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  7. Takahiro Kusakabe
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  8. Jae Man Lee
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Correspondence to Jae Man Lee.

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Xu, J., Yoshimura, K., Mon, H. et al. Establishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells. Mol Biotechnol 56, 193–198 (2014). https://doi.org/10.1007/s12033-013-9694-0

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  • DOI: https://doi.org/10.1007/s12033-013-9694-0

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