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Genetic Diversity of ‘Ubá’ Mango Tree Using ISSR Markers

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Abstract

In this study, the genetic diversity of ‘Ubá’ mango trees cultivated at the Zona da Mata of Minas Gerais State, Brazil, was assessed, to identify whether there is variability in the plants grown in the region, justifying the mass selection as a breeding method. We used 102 accessions. Leaves were collected for extraction of genomic DNA, which was amplified with nine ISSR primers. The data obtained by the analysis of electrophoretic patterns were arranged in a binary matrix, considering 0 for the absence and 1 for the presence of bands. Based on these data, we performed the analysis of genetic dissimilarity and carried out the cluster analysis by the methods of Tocher and graphical dispersion. The most similar accessions are 144 and 150, both coming from Ubá, while the most divergent ones are 29 and 97, from Visconde do Rio Branco. The grouping by the Tocher method separated the accessions into six groups, 94.1% of which were allocated in the first group and showed that there is no separation of accessions depending on the sampling sites. The 3D scatter plot reinforces this conclusion. There is genetic variability among the accessions of ‘Ubá’ mango tree evaluated. Therefore, it is possible to make mass selection in open-pollinated populations.

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Acknowledgments

The authors are grateful to the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) for the scholarship granted and the financial support provided for the ‘Ubá’ Mango Tree Breeding Project. The authors are also grateful to the Laboratório de Biologia Molecular of the Departamento de Biologia Geral of the UFV for the support extended to them during the molecular analyses.

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Authors and Affiliations

  1. Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, Campus Petrolina Zona Rural, Petrolina, PE, Brazil

    Aline Rocha

  2. Departamento de Fitotecnia, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, Viçosa, MG, CEP 36570-000, Brazil

    Luiz Carlos Chamhum Salomão & Dalmo Lopes de Siqueira

  3. Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Tânia Maria Fernandes Salomão & Cosme Damião Cruz

Authors
  1. Aline Rocha
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  2. Luiz Carlos Chamhum Salomão
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  3. Tânia Maria Fernandes Salomão
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  4. Cosme Damião Cruz
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  5. Dalmo Lopes de Siqueira
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Correspondence to Luiz Carlos Chamhum Salomão.

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Rocha, A., Salomão, L.C.C., Salomão, T.M.F. et al. Genetic Diversity of ‘Ubá’ Mango Tree Using ISSR Markers. Mol Biotechnol 50, 108–113 (2012). https://doi.org/10.1007/s12033-011-9419-1

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