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Probing of l-Arginine as an Additive for the Temperature-Induced Aggregation of Veterinary Growth Hormones: Fluorescence Study

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Abstract

The influence of l-arginine on the temperature-induced aggregation of porcine and mink growth hormones was studied by fluorescence spectroscopy. It was found that l-arginine suppresses the heat-induced aggregation. Moreover, the analysis of l-arginine interaction with the native proteins by fluorescence spectroscopy and circular dichroism spectroscopy revealed no significant changes in their native structure. On the basis of the results, l-arginine could be considered as a potential additive for the prevention of storage and temperature-related denaturation and aggregation of veterinary growth hormones.

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  1. Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Sauletekio al. 11, LT-10223, Vilnius-40, Lithuania

    Andrejus Cirkovas & Jolanta Sereikaite

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  1. Andrejus Cirkovas
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  2. Jolanta Sereikaite
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Correspondence to Jolanta Sereikaite.

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Cirkovas, A., Sereikaite, J. Probing of l-Arginine as an Additive for the Temperature-Induced Aggregation of Veterinary Growth Hormones: Fluorescence Study. Mol Biotechnol 49, 11–18 (2011). https://doi.org/10.1007/s12033-010-9370-6

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