Cell-Based Assays for High-Throughput Screening

Abstract

Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.

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Acknowledgments

We thank Drs. M. Schenone and I. Smukste for insightful discussions on target identification. The study was funded in whole or in part with federal funds from the National Cancer Institute’s Initiative for Chemical Genetics, National Institutes of Health, under Contract no. N01-CO-12400.

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Correspondence to Nicola Tolliday.

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An, W.F., Tolliday, N. Cell-Based Assays for High-Throughput Screening. Mol Biotechnol 45, 180–186 (2010). https://doi.org/10.1007/s12033-010-9251-z

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Keywords

  • Assay development
  • Cell-based assay
  • High-content screening (HCS)
  • High-throughput screening (HTS)
  • Small-molecule screening
  • Target identification