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One Novel Mitochondrial Citrate Synthase from Oryza sativa L. can Enhance Aluminum Tolerance in Transgenic Tobacco

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Abstract

Rice exhibits the greatest aluminum (Al) tolerance compared with other cereals such as wheat, barley, maize, etc. A full-length gene, OsCS1, encoding citrate synthase, which is highly induced by aluminum toxicity in rice (Oryza sativa L.), was isolated. Sequence analysis and the sub-cellular localization of OsCS1 in yeast revealed that it is a mitochondrial citrate synthase. OsCS1 was induced by Al toxicity. Several independent transgenic tobacco lines expressing OsCS 1 exhibitted increased citrate efflux and extraordinary Al tolerance. Possible outlook for OsCS1 to be applied to enhance plant tolerance to Al toxicity was also discussed.

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Acknowledgment

The study was supported by the National Science Foundation of China (NSFC), No. 30570988, the Program for New Century Excellent Talents in University, No. NCET- 07- 020, and the Youth Science and Technology Phosphor Foundation of Shanghai (08QH14003).

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, 220 Handan Road, Shanghai, 200433, China

    Yingying Han, Wenzheng Zhang, Bailong Zhang, Shanshan Zhang, Wei Wang & Feng Ming

  2. Institute of Plant Biology, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, China

    Yingying Han, Wenzheng Zhang, Bailong Zhang, Shanshan Zhang, Wei Wang & Feng Ming

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  1. Yingying Han
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  2. Wenzheng Zhang
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Correspondence to Feng Ming.

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Han, Y., Zhang, W., Zhang, B. et al. One Novel Mitochondrial Citrate Synthase from Oryza sativa L. can Enhance Aluminum Tolerance in Transgenic Tobacco. Mol Biotechnol 42, 299–305 (2009). https://doi.org/10.1007/s12033-009-9162-z

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