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Genetic Diversity of Hevea IRRDB’81 Collection Assessed by RAPD Markers

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Abstract

The majority of Hevea (Hevea brasiliensis Muell. Arg.) genetic resource in Vietnam derived from the IRRDB’81 germplasm collected in the Amazonian habitats of the genus. Random amplified polymorphic DNA (RAPD) analysis was used to examine the genetic diversity and structure of the IRRDB’81 germplasm. A total of 59 accessions from 13 different districts of the Brazilian states namely Acre, Rondonia, and Mato Grosso were brought into the study using six arbitrarily preselected primers. Sixty-five RAPD band patterns ranging in size from 0.2 to 3.0 kbp were scored for analysis. Differences in the level of DNA polymorphism among the districts and states were revealed. The percentage of the polymorphic DNA fragments calculated for 13 individual districts varied from 15.38 to 70.77%. The mean values of heterozygosity within the district varied from 0.064 to 0.264. Pairwise district Nei’s genetic distance values ranged from 0.046 for Catriquacu and Itanba of Mato Grosso to 0.304 for Tarauaca of Acre and Aracatuba of Mato Grosso. The estimated values of Shannon’s diversity index ranged from 0.093 for the Assis-Brasil district of Acre to 0.389 for the Jiparana district of Rondonia. The analysis of molecular variance (AMOVA) indicated that most of the genetic variations were found among accessions within the districts, while interdistrict variance component accounted for 14.1% only. The low interdistrict differentiation probably implied an extensive gene flow among them. Both the principal coordinate analysis and UPGMA cluster analysis based on genetic distance values revealed a varying degree of separation among the districts and that conformed to geographical origins of Hevea IRRDB’81 collection.

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Acknowledgments

The research was supported by grant from the Rubber Research Institute of Vietnam. The authors would like to express their gratitude to Mr. Mai Van Son, Director of Rubber Research Institute of Vietnam for his permission to present this paper. Thanks also go to the officers and staffs of Breeding Division of Rubber Research Institute of Vietnam for their contribution to this research.

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  1. Rubber Research Institute of Vietnam, 301 Nguyen Van Troi Street, Tan Binh District, Ho Chi Minh City, Vietnam

    Lai Van Lam, Tran Thanh, Vu Thi Quynh Chi & Le Mau Tuy

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  1. Lai Van Lam
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Correspondence to Tran Thanh.

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Lam, L.V., Thanh, T., Chi, V.T.Q. et al. Genetic Diversity of Hevea IRRDB’81 Collection Assessed by RAPD Markers. Mol Biotechnol 42, 292–298 (2009). https://doi.org/10.1007/s12033-009-9159-7

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  • DOI: https://doi.org/10.1007/s12033-009-9159-7

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