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Study on the Spatial Architecture of p53, MDM2, and p14ARF Containing Complexes

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Abstract

We have developed a surface plasmon resonance (SPR)-based immunocapture approach to study multimeric protein–protein complexes. A composition and spatial architecture of protein complexes that contained GST-tagged p53, p14ARF, and MDM2 was examined by the developed approach. Obtained results verified that the p53 protein possesses two binding sites for MDM2. Ternary complexes containing p14ARF, MDM2, and p53 proteins could only be formed when MDM2 protein functions as a bridging molecule. That was confirmed by immunoprecipitation and immunostaining.

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Acknowledgments

Swedish Cancer Society, a matching grant from the Concern Foundation (Los Angeles), the Cancer Research Institute (New York), Swedish Institute, and Swedish Foundation for Strategic Research supported this work.

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Authors and Affiliations

  1. V. Lashkaryov Institute of Semiconductor Physics, NASU, 42 Prospekt Nauki, 03 028, Kyiv, Ukraine

    Andrej Savchenko & Boris Snopok

  2. R. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, 45 Vasylkivska Street, 03 022, Kyiv, Ukraine

    Mariya Yurchenko

  3. Department of Microbiology, Tumor, and Cell Biology (MTC), Karolinska Institute, S 171 77, Stockholm, Sweden

    Elena Kashuba

  4. IRIS (Center for Integrative Recognition in the Immune System), Karolinska Institute, S 171 77, Stockholm, Sweden

    Elena Kashuba

Authors
  1. Andrej Savchenko
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  2. Mariya Yurchenko
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  3. Boris Snopok
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  4. Elena Kashuba
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Corresponding author

Correspondence to Elena Kashuba.

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Andrej Savchenko and Mariya Yurchenko have contributed equally to this article.

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Savchenko, A., Yurchenko, M., Snopok, B. et al. Study on the Spatial Architecture of p53, MDM2, and p14ARF Containing Complexes. Mol Biotechnol 41, 270–277 (2009). https://doi.org/10.1007/s12033-008-9116-x

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  • DOI: https://doi.org/10.1007/s12033-008-9116-x

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