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Gene Expression Profiles of Mouse Striatum in Control and Maneb + Paraquat-induced Parkinson’s Disease Phenotype: Validation of Differentially Expressed Energy Metabolizing Transcripts

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Abstract

The present study was undertaken to investigate the gene expression patterns of the striatum of control and maneb + paraquat-induced Parkinson’s disease (PD) phenotype in mouse to identify the differentially expressed transcripts. The animals were treated with and without maneb (30 mg/kg, i.p.) + paraquat (10 mg/kg, i.p.), twice a week, for 3, 6, and 9 weeks. The RNA was isolated from control and treated mouse striatum and reverse transcribed, and equal quantities of labeled cDNA were mixed and hybridized with mouse 15 k arrays. Comparative transcription patterns showed the time of exposure dependent alteration in the expression of several transcripts associated with various pathways. RT-PCR reconfirmed the differential expression of some energy metabolizing transcripts. The study provides maneb + paraquat-induced differential expression of many transcripts using high-density microarray approach. Few transcripts, which were previously not reported to be associated with neuronal degeneration, were also identified. The results obtained thus suggest that maneb + paraquat induce neurotoxicity in the striatum in a time of exposure dependent manner via multiple pathways and defective energy metabolism could play a critical role.

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Acknowledgments

The authors sincerely thank the Council of Scientific and Industrial Research (CSIR), New Delhi, and University Grant Commission, New Delhi, for providing research fellowship to Suman Patel and Seema Singh, respectively. The authors also thank Department of Biotechnology (DBT), India, for providing financial support for the study. The ITRC communication number of this manuscript is 2580.

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  1. Industrial Toxicology Research Centre (ITRC), Mahatma Gandhi Marg, P. O. Box 80, Lucknow, 226 001, India

    Suman Patel, Kavita Singh, Seema Singh & Mahendra Pratap Singh

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  1. Suman Patel
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  2. Kavita Singh
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  3. Seema Singh
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  4. Mahendra Pratap Singh
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Correspondence to Mahendra Pratap Singh.

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Patel, S., Singh, K., Singh, S. et al. Gene Expression Profiles of Mouse Striatum in Control and Maneb + Paraquat-induced Parkinson’s Disease Phenotype: Validation of Differentially Expressed Energy Metabolizing Transcripts. Mol Biotechnol 40, 59–68 (2008). https://doi.org/10.1007/s12033-008-9060-9

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