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Effect of insulin on IR and GLP1-R expressions in HT22 cells

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Abstract

Insulin is a significant growth factor that specifically binds to the insulin receptor (IR) in the brain and then activates the PI3K-AKT pathway. Glucagon-like peptide 1 (GLP-1) has a variety of functions including neuroprotection, support for neurogenesis, and increasing insulin signal. This study aims to investigate the effect of insulin administered to immortalized clonal mouse hippocampal cell line (HT22) at different doses and intervals on IR, insulin receptor A (IRA), insulin receptor B (IRB), and Glucagon-like peptide 1 receptor (GLP1-R) mRNA expression and protein levels. The cells were planted in 6 well plates at a density of 3 × 105/4 × 105. Cells treated with insulin at different concentrations (5, 10, and 40 nM) were collected at 0.5, 2, 8, 16, and 24 h. RT-PCR and western blot analysis were used to measure mRNA expression and protein levels. Our results showed that insulin has short and long-term effects on IR and GLP1-R expression depending on dose and time. These findings may guide future studies targeting IR isoforms and GLP1-R in particular, as well as determining the optimal dose and duration of insulin stimulation in insulin signaling research.

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No data associated in the manuscript.

Abbreviations

IR:

Insulin receptor

GLP-1:

Glucagon-like peptide 1

HT22:

Mouse hippocampal cell line

GLP1-R:

Glucagon-like peptide 1 receptor

IRA:

Insulin receptor A

IRB:

Insulin receptor B

RT-qPCR:

Real-time quantitative PCR

PBS:

Phosphate-buffered saline

RIPA:

Radioimmunoprecipitation assay buffer

PVDF:

Polyvinylidene difluoride

PBST:

PBS-Tween

MALAT1:

Metastatic-related pulmonary adenocarcinoma transcript 1

SRSF2:

Reducing factor cool-arginine-rich fusion factor 2

PAK2:

P21-activated kinases

PTZ:

Pentylentetrazole

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Funding

This study is supported by funding from the Pamukkale University (Project number 2022HZDP013).

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey

    Melek Tunc-Ata & Vural Kucukatay

  2. Department of Neuroscience, School of Medicine, University of Connecticut, Farmington, CT, USA

    Zeynep Mine Altunay

  3. Department of Neuroscience, Institute of Health Sciences, Pamukkale University, Denizli, Turkey

    Aysel Alphan

Authors
  1. Melek Tunc-Ata
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  2. Zeynep Mine Altunay
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  3. Aysel Alphan
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  4. Vural Kucukatay
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Contributions

MTA, ZMA, AA and VK: Conceptualization, MTA, ZMA and VK: Data curation, ZMA and VK: Formal analysis, VK and AA: Funding acquisition, MTA, ZMA, AA and VK: Investigation, ZMA and AA: Methodology, VK: Supervision, MTA: Validation, ZMA: Visualization, MTA, ZMA and VK: Writing—original draft, MTA and VK: Reviewing. All authors have read and agree to the published version of the manuscript.

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Correspondence to Melek Tunc-Ata.

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Tunc-Ata, M., Altunay, Z.M., Alphan, A. et al. Effect of insulin on IR and GLP1-R expressions in HT22 cells. Med Oncol 40, 301 (2023). https://doi.org/10.1007/s12032-023-02172-w

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