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METTL3 regulatory TROAP can regulate the progression of non-small cell lung cancer through PI3K/AKT and EMT signaling pathway

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Abstract

TROAP, interacts with trophinin and bystin, polys a key role in embryo implantation. TROAP is required for spindle assembly and centrosome integrity during the mitosis. TROAP has been described to promote tumorigenesis in a diverse range of cancer. We performed this study to assess the biological and clinical significance of TROAP in Non-small cell lung cancer. Forty-eight pairs of lung adenocarcinoma (LUAD) tissues and paraneoplastic tissues were collected. RT-qPCR, western bolt and immunohistochemistry assay was used to test TROAP RNA and protein expression not in LUAD tissues and paraneoplastic tissues but in LUAD cell lines and control cell lines. TROAP knockdown and overexpression vector were constructed and transfected into lung cancer cells. CCK-8, transwell, and wound healing assays were used to assess cell viability, migration, and invasion. The expression of PI3K/AKT and EMT signaling proteins and METTL3 were determined by western blot. We found the TROAP was enriched in NSCLC tissues and cell lines. TROAP knockdown inhibited cell proliferation, migration, invasion compared with control group in NSCLC. Mechanism analysis revealed that TROAP activated PI3K/AKT and EMT signaling pathway. To a certain extent, TROAP was regulated by METTL3. In a word, TROAP accelerated the progression of NSCLC through PI3K/AKT and EMT pathway, and TROAP might be considered as a novel target for NSCLC therapy.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

Funding was provided by National Natural Science Foundation of China (Nos. 81760383, 82060385) and The Graduate Innovation Fund of Kunming Medical University in 2022 (No. 2022S067).

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Authors and Affiliations

  1. Department of Clinical Laboratory, Yunnan Molecular Diagnostic Center, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China

    Muli Xu, Xiaoxiao Liu, Wanting Jia, Yu Duan, Di Ma, Jiaxuan Ma, Wanyang Lei & Wenlin Tai

  2. Chinese Academy of Medical Sciences and Institute of Medical Biology, Peking Union Medical College, Kunming, China

    Jiankun Yu

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  1. Muli Xu
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Correspondence to Wenlin Tai.

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The study was approved by Ethical Committee of the Third Affiliated Hospital of Kunming Medical University and conducted in accordance with the ethical standards.

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Xu, M., Yu, J., Liu, X. et al. METTL3 regulatory TROAP can regulate the progression of non-small cell lung cancer through PI3K/AKT and EMT signaling pathway. Med Oncol 40, 274 (2023). https://doi.org/10.1007/s12032-023-02143-1

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