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Investigations on effects of titanium dioxide (TiO2) nanoparticle in combination with UV radiation on breast and skin cancer cells

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Abstract

In this study, we have investigated the chemotherapeutic potential of titanium dioxide (TiO2) nanoparticles on skin and breast cancer cells. The cells have treated with a 75 µg/ml concentration of titanium dioxide because it is a recommended dose with proven effectiveness in vitro studies and then the cells were exposed to UV-A radiation. The combined effects of titanium dioxide and UV-A radiation on cell viability, cell cycle, plasma membrane, mitochondrial membrane potentials and apoptotic activity of the cells were investigated. The viability of SK-MEL 30 cells was measured by MTT assay and apoptotic activity of cells was determined by Annexin-V FITC/PI staining. As a result of the research, an increase was observed in the viability of cells treated with 75 µg/ml titanium dioxide concentration, while a significant decrease in cell viability was observed for both cell types when UV-A radiation and TiO2 were applied together. The results also showed that the percentage of apoptotic cells increased as a result of UV + TiO2 exposure. Accordingly, it can be said that TiO2 nanoparticles may research as potential chemotherapeutic agents for skin and breast cancers, especially in the presence of UV radiation.

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Data availability

The datasets generated and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.

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Funding

This study was funded by the Gazi University BAP department (Grant No: 01/2019-32).

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Tokat Gaziosmanpaşa University, Tokat, Turkey

    Mustafa Tuğfan Bilkan

  2. Department of Physiology, Faculty of Gülhane Medicine, Sağlık Bilimleri University, Ankara, Turkey

    Zehra Çiçek & Mehmet Özler

  3. Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey

    Ayşe Gülnihal Canseven Kurşun & Meriç Arda Eşmekaya

Authors
  1. Mustafa Tuğfan Bilkan
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  2. Zehra Çiçek
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  3. Ayşe Gülnihal Canseven Kurşun
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  4. Mehmet Özler
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  5. Meriç Arda Eşmekaya
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Contributions

Conceptualization: MAE, MTB; methodology: MAE, MTB, AGCK; formal analysis and investigation: MAE, ZÇ; writing–original draft preparation: MAE, MTB; writing–review and editing: MAE, MTB; funding acquisition: MAE; resources: MAE, ZÇ, MÖ; supervision: MAE.

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Correspondence to Mustafa Tuğfan Bilkan.

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Bilkan, M.T., Çiçek, Z., Kurşun, A.G.C. et al. Investigations on effects of titanium dioxide (TiO2) nanoparticle in combination with UV radiation on breast and skin cancer cells. Med Oncol 40, 60 (2023). https://doi.org/10.1007/s12032-022-01931-5

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