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CD44-positive cancer stem cells from oral squamous cell carcinoma exhibit reduced proliferation and stemness gene expression upon adipogenic induction

Medical Oncology volume 39, Article number: 23 (2022) Cite this article

Abstract

We proposed to assess adipogenic differentiation and its effect on the proliferation and stemness markers in CD44 + OSCC CSCs. D44 + CSCs were sorted by magnetic sorting from the single-cell suspension of the OSCC tumor. Adipogenic differentiation was induced by an adipogenic induction medium. Lipid droplet formation was confirmed by oil red O staining. The expression of the cell surface marker was analyzed by flow cytometry. Real-time qPCR was performed to examine the gene expression activity. CD44 + OSCC CSCs can differentiate into adipocytes and adipogenesis in these cells decrease their proliferation and stemness gene expression. Adipogenic induction can make the cancer stem cells from OSCC tumors lose their stemness potential. Oral cancer, especially OSCC, is a huge burden worldwide. Similar to other stem cells, cancer stem cells can differentiate into other lineage cells. Our study shows that the proliferation and stemness gene expression in the CSCs from OSCC tumors can be thwarted by inducing them to differentiate into adipocytes, which could be advantageous to find out new clinical approaches in the treatment of cancers, like OSCC.

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Funding

The present work was funded by the Taif University Researchers Supporting Program (Project number: TURSP-2020/151), Taif University, Saudi Arabia.

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Authors and Affiliations

  1. Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, 45412, Saudi Arabia

    Shankargouda Patil

  2. Department of Human Anatomy, College of Medicine, Taif University, Taif, 21944, Saudi Arabia

    Ashraf Al-Brakati

  3. Department of Oral and Maxillofacial Surgery, College of Dentistry, Jazan University, Jazan, 45412, Saudi Arabia

    Nazim H. Abidi

  4. Department of Oral and Maxillofacial Surgery, King Abdulaziz University, Jeddah city, Saudi Arabia

    Mazen A. Almasri

  5. Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, Riyadh Elm University, Riyadh, 12611, Saudi Arabia

    Asma saleh Almeslet

  6. Biogenre Private Limited, Pune, 412105, India

    Vikrant R. Patil

  7. Department of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai, 600130, India

    A. Thirumal Raj

  8. Department of Restorative Dental Science, Division of Operative Dentistry, College of Dentistry, Jazan University, Jazan, 45142, Saudi Arabia

    Shilpa Bhandi

Authors
  1. Shankargouda Patil
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  2. Ashraf Al-Brakati
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  6. Vikrant R. Patil
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Correspondence to Shankargouda Patil.

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The study was conducted according to the guidelines of the Declaration of Helsinki. The research protocol was approved by Scientific Research, College of Dentistry, Jazan University. Reference Number: CODJU-19212.

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Patil, S., Al-Brakati, A., Abidi, N.H. et al. CD44-positive cancer stem cells from oral squamous cell carcinoma exhibit reduced proliferation and stemness gene expression upon adipogenic induction. Med Oncol 39, 23 (2022). https://doi.org/10.1007/s12032-021-01617-4

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  • DOI: https://doi.org/10.1007/s12032-021-01617-4

Keywords

  • Adipogenic differentiation
  • Cancer stem cells
  • CD44
  • OSCC
  • Stemness