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Role of Src/FAK in migration and invasion mediated by extracellular vesicles from MDA-MB-231 cells stimulated with linoleic acid

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Abstract

Linoleic acid (LA) is the most abundant polyunsaturated fatty acid in occidental diets, which mediate a variety of processes in human breast cancer cells, including migration and invasion. Extracellular vesicles (EVs) are vesicles released from endosomes and plasma membrane that are composed of a variety of molecules, including proteins, nucleic acids and lipids. EVs from cancer cells promote processes related with cancer progression. In the present study, we demonstrate that treatment of MDA-MB-231 cells with EVs from MDA-MB-231 cells stimulated with LA (LA EVs) promote migration and invasion via Src activity. LA EVs induce activation of FAK via Src activity and of Src and Akt2. LA EVs also induce the assembly of focal adhesions and MMP-9 secretion. These findings demonstrate that LA EVs mediate an autocrine and/or paracrine Src/FAK signaling pathway to promote migration and invasion.

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All data generated or analyzed during this study are included in this article.

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Acknowledgements

We are grateful to Nora Ruiz and María de Lourdes-Rojas (LaNSE, Cinvestav-IPN) for their technical assistance.

Funding

This research was funded by CONACYT (255429) and CONACYT-FOSISS (Salud 2015-1-261637), Mexico. Grants from CONACYT supported JRR, ELO, AGH and RDA.

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Authors and Affiliations

  1. Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, 07360, Mexico City, Mexico

    Javier Ramirez-Ricardo, Elizabeth Leal-Orta, Alejandra Garcia-Hernandez, Ricardo Diaz-Aragon, Pedro Cortes-Reynosa & Eduardo Perez Salazar

  2. Hospital 1º de Octubre-ISSSTE, Mexico City, Mexico

    Rocio Thompson-Bonilla

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  1. Javier Ramirez-Ricardo
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  2. Elizabeth Leal-Orta
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  7. Eduardo Perez Salazar
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Contributions

JR-R designed the study and wrote the manuscript. JR-R and EL-O performed the experiments and analyses. AG-H, RD-A and PC-R validated the data and reviewed/edited the manuscript. EPS, RT-B and JR-R coordinated the study and wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Eduardo Perez Salazar.

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12032_2021_1485_MOESM1_ESM.tif

Fig. S1. Schematic diagram showing the experimental procedure of stimulation of MDA-MB-231 with 90 μM LA for different periods of time (12, 24, 36 and 48 h). (TIF 1120 kb)

12032_2021_1485_MOESM2_ESM.tif

Fig. S2. Schematic diagram showing the experimental procedure of treatment for 6, 9, 12, 18 and/or 24 h of MDA-MB-231 cells with Ctrl EVs and LA EVs. (TIF 1436 kb)

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Ramirez-Ricardo, J., Leal-Orta, E., Garcia-Hernandez, A. et al. Role of Src/FAK in migration and invasion mediated by extracellular vesicles from MDA-MB-231 cells stimulated with linoleic acid. Med Oncol 38, 40 (2021). https://doi.org/10.1007/s12032-021-01485-y

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