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Carboxyl-terminal polypeptide fragment of MUC16 combing stathmin1 promotes gallbladder cancer cell migration and invasion

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Abstract

CA-125, coded by MUC16 gene, is responsible to many kinds of tumor metastasis. However, the related mechanism remains unclear. We have established a novel manner to reveal gallbladder cancer metastasis related to serum CA-125 levels through the C-terminal polypeptide of MUC16 gene production. MUC16 C-terminal polypeptide significantly promoted gallbladder cancer cell migration and invasion in vitro. Mass spectrum indicated that interaction of MUC16 C-terminal fragment with the C-terminal domain of stathmin1 inhibited the phosphorylation of stathmin1 to promote the combination with tubulin. Stathmin1 knockdown inhibited the migration and invasion of gallbladder cancer cells in vitro and lung metastasis in vivo induced by MUC16 C-terminal polypeptide. The high expression level of MUC16c consistent with stathmin1 was also confirmed in gallbladder cancer tissues. Our study revealed the underlying mechanism of gallbladder cancer metastasis related to CA-125 levels, which was beneficial for CA-125 in gallbladder cancer diagnose and therapy.

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Funding

This work was supported by the National Natural Science Foundation of China Grants (81802751), JianFeng Project of XuHui Provincial Commission of Health and Family Planning (SHXH201703), and Shanghai Municipal Natural Science Foundation (16411952000). All authors have contributed to this study.

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Author notes

  1. Kun Fan, Dexiang Zhang, and Min Li have been contributed equally to this article.

Authors and Affiliations

  1. General Surgery Department, Zhongshan-Xuhui Hospital Affiliated to Fudan University, Shanghai, China

    Kun Fan, Dexiang Zhang & Houbao Liu

  2. Department of General Surgery, Zhongshan Hospital, Fudan University, 180, Fenglin Road, Xuhui, Shanghai, China

    Kun Fan, Min Li, Sheng Shen, Jiwen Wang, Xiaojian Ni, Zijun Gong, Bohao Zheng, Zhihui Gao, Xiaoling Ni, Tao Suo, Han Liu & Houbao Liu

  3. Biliary Tract Disease Center of Zhongshan Hospital, Fudan University, Shanghai, China

    Kun Fan, Min Li, Sheng Shen, Jiwen Wang, Xiaojian Ni, Zijun Gong, Bohao Zheng, Zhihui Gao, Xiaoling Ni, Tao Suo, Han Liu & Houbao Liu

  4. Biliary Tract Disease Institute, Fudan University, Shanghai, China

    Kun Fan, Min Li, Sheng Shen, Jiwen Wang, Xiaojian Ni, Zijun Gong, Bohao Zheng, Zhihui Gao, Xiaoling Ni, Tao Suo, Han Liu & Houbao Liu

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  1. Kun Fan
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  2. Dexiang Zhang
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  10. Xiaoling Ni
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  11. Tao Suo
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  12. Han Liu
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Correspondence to Tao Suo, Han Liu or Houbao Liu.

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Fan, K., Zhang, D., Li, M. et al. Carboxyl-terminal polypeptide fragment of MUC16 combing stathmin1 promotes gallbladder cancer cell migration and invasion. Med Oncol 37, 114 (2020). https://doi.org/10.1007/s12032-020-01438-x

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