Induction of apoptosis of liver cancer cells by nanosecond pulsed electric fields (nsPEFs)
- 460 Downloads
The application of nanosecond pulsed electric fields (nsPEFs) is a novel method to induce the death of cancer cells. NsPEFs could directly function on the cell membrane and activate the apoptosis pathways, then induce apoptosis in various cell lines. However, the nsPEFs-inducing-apoptosis action sites and the exact pathways are not clear now. In this study, nsPEFs were applied to the human liver cancer cells HepG2 with different parameters. By apoptosis assay, morphological observation, detecting the mitochondrial membrane potential (ΔΨ m), intracellular calcium ion concentration ([Ca2+]i) and the expressions of key apoptosis factors, we demonstrated that nsPEFs could induce the morphology of cell apoptosis, the change in ΔΨ m, [Ca2+]i and the upregulation of some key apoptosis factors, which revealed the responses of liver cancer cells and indicated that cells may undergo apoptosis through the mitochondria-dependent pathway after nsPEFs were applied.
KeywordsCellular morphology Mitochondrial membrane potential Calcium Electric pulses HepG2
This study was sponsored by Project No. 106112015CDJZR158804 supported by the Fundamental Research Funds for the Central Universities.
Compliance with ethical standards
Conflict of interest
All the authors declare that they have no conflict of interest.
No human subjects or animals were used in this study.
- 22.Vernier PT, Li A, Marcu L, Craft CM, Gundersen MA. Ultrashort pulsed electric fields induce membrane phospholipid translocation and caspase activation: differential sensitivities of Jurkat T lymphoblasts and rat glioma C6 cells. IEEE Trans Dielectr Electr Insul. 2003;10:795–809.CrossRefGoogle Scholar
- 28.Beebe SJ, Ford WE, Ren W, Chen X, Schoenbach KH. Non-ionizing radiation with nanosecond pulsed electric fields as a cancer treatment: in vitro studies. IEEE Eng Med Biol Soc Conf. 2009;2009:6509–12.Google Scholar