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Medical Oncology

, 34:21 | Cite as

RNAi-mediated knockdown of MCM7 gene on CML cells and its therapeutic potential for leukemia

  • Liang Tian
  • Juan Liu
  • Guo-Hua Xia
  • Bao-An ChenEmail author
Original Paper

Abstract

MCM7 is one of the subunits of MCM2-7 complex, which is essential to DNA replication licensing and the control of cell cycle progression. It has been demonstrated that MCM7 participates in mRNA transcription and DNA damage regulation as well. MCM7 gene is found to be over-expressed in multiple cancers, but there are few reports about its effect in leukemia. Recent studies have proven that MCM7 expression has a relationship with diagnosis and prognosis, which has led to their potential clinical application as a marker for cancer screening. RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. It is a valuable research tool, which is widely used in cell culture and living organisms as well as in medicine recent years. It is indicated that RNAi application for targeting functional carcinogenic molecules, tumor resistance to chemotherapy and radiotherapy is required in cancer treatment. Gene products knockdown by RNAi technology exerts anti-proliferative and pro-apoptotic effects upon cell culture systems, animal models and in clinical trials in the most studies. In the present study, we found that MCM7 highly expressed in K562 cells rather than that in normal neutrophils. Thus, lentivirus-mediated shRNA targeting MCM7 was used to suppress its endogenous expression in K562 cells and develop a novel therapeutic strategy for leukemia.

Keywords

MCM7 gene Lentivirus-mediated siRNA K562 cell Leukemia 

Notes

Acknowledgements

This study was funded by the Fundamental Research Funds for the Central Universities and Colleges and Universities in Jiangsu Province plans to graduate research and innovation 2014 (No. KYLX_0198); Key Medical Projects of Jiangsu Province (BL2014078).

Compliance with ethical standards

Conflict of interest

None.

Ethics approval and consent to participate

All animal experiments were conducted in conformity with institutional guidelines for the care and use of laboratory animals, and protocols were approved by the National Science Council, Republic of China, and the Institutional Animal Care and Use Committee in Southeast University, China (NO. 2007-51).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Liang Tian
    • 1
  • Juan Liu
    • 2
  • Guo-Hua Xia
    • 3
  • Bao-An Chen
    • 1
    Email author
  1. 1.Department of Hematology and Oncology, Medical School, Zhongda HospitalSoutheast UniversityNanjingChina
  2. 2.Department of Digestive System, Medical School, Zhongda HospitalSoutheast UniversityNanjingChina
  3. 3.Department of Clinical Laboratory, Medical SchoolSoutheast UniversityNanjingChina

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