Micro-RNA (miRNA) profile in Hodgkin lymphoma: association between clinical and pathological variables
- 536 Downloads
miRNAs are small RNAs and control the expression of protein-encoding genes. The aim of this study was to determine the association between miRNA profile and clinical variables including age, stage, B symptom, histopathologic subtype, response to treatment, disease-free survival (DFS) and overall survival (OS) in classical Hodgkin lymphoma (cHL). A total of 377 miRNAs were studied by qPCR in 32 cases with cHL, and results were compared with 60 samples taken from cases with reactive lymphadenopathy. Biogazelle qbasePLUS 2.0 software was used to analyze the results. miR-582-3p, miR-525-3p, miR-448, miR-512-3p, miR-642a-5p, miR-876-5p, miR-532-3p, miR-654-5p, miR-128, miR-145-5p, miR-15b-5p, miR-328 and miR-660-5p were found to be decreased in cHL compared with controls. In contrast, miR-34a-5p (2.626-fold), miR-146a-5p (4.32-fold), miR-93-5p (2.347-fold), miR-20a-5p (4.930-fold), miR-339-3p (4.948-fold), miR-324-3p (4.98-fold), miR-372 (7.038-fold), miR-127-3p (8.234-fold), miR-155-5p (4.947-fold), miR-320a (17.502-fold) and miR-370 (21.479-fold) (p < 0.05) were found to be increased in cHL. There was no difference in miRNA profile according to the age, sex, stage, response to treatment, DFS and OS. However, miR-889 was found to be increased in patients with B symptom and miR-127-3p was found to be increased in nodular sclerosing subtype. Some miRNAs increase and some decrease in cHL. However, there was no clinical association between clinical variables and with the majority of the miRNA profile studied in this study. miR-889 and miR-127-3p were related to B symptom and nodular sclerosis subtype, respectively. We need more studies evaluating miRNA profile and clinical outcome in Hodgkin Lymphoma.
KeywordsHodgkin lymphoma Subtype miRNA Prognosis Clinicopathological correlation Survival qPCR B symptom
This study has been supported by Turkish Society of Hematology.
Semra Paydas contributed to study design, clinical management of the patients, paper writing and last control. Arbil Acikalin-Melek Ergin contributed to histopathologic examinations and re-evaluations of the biopsy samples; Hikmet Celik-Basak Yavuz did PCR analyses; Kahraman Tanriverdi contributed to coordination of all laboratory studies and statistical analyses.
Compliance with ethical standards
Conflict of interest
There is no conflict of interest.
This study has been approved by the ethics committee.
Informed consent was obtained from all individual participants included in the study.
- 3.Ji J, Shi J, Budhu A, Yu Z, Forgues M, Roessler S, Ambs S, Chen Y, Meltzer PS, Croce CM, Qin LX, Man K, Lo CM, Lee J, Ng IO, Fan J, Tang ZY, Sun HC, Wang XW. MicroRNA expression, survival, and response to interferon in liver cancer. N Engl J Med. 2009;361:1437–47.CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O’Briant KC, Allen A, Lin DW, Urban N, Drescher CW, Knudsen BS, Stirewalt DL, Gentleman R, Vessella RL, Nelson PS, Martin DB, Tewari M. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA. 2008;105:10513–8.CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Van Vlierberghe P, De Weer A, Mestdagh P, Feys T, De Preter K, De Paepe P, Lambein K, Vandesompele J, Van Roy N, Verhasselt B, Poppe B, Speleman F. Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs. Br J Haematol. 2009;147:686–90.CrossRefPubMedGoogle Scholar
- 13.Nie K, Gomez M, Landgraf P, Garcia JF, Liu Y, Tan LHC, Chadburn A, Tuschl T, Knowles DM, Tam W. MicroRNA-Mediated Down-Regulation of PRDM1/Blimp-1in Hodgkin/Reed-Sternberg cells: a potential pathogenetic lesion in Hodgkin lymphomas. Am J Pathol. 2008;173:242–52.CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Sanchez-Espiridion B, Martin-Moreno AM, Montalban C, Figueroa V, Vega F, Younes A, Medeiros LJ, Alves FJ, Canales M, Estevez M, Menarguez J, Sabin P, Ruiz-Marcellan MC, Lopez A, Sanchez-Godoy P, Burgos F, Santonja C, Lopez J, Piris MA, Garcia JF. MicroRNA signatures and treatment response in patients with advanced classical Hodgkin lymphoma. Br J Haematol. 2013;162:336–47.CrossRefPubMedGoogle Scholar