Abstract
Among noncoding RNAs, microRNAs (miRNAs) have been most extensively studied, and their biology has repeatedly been proven critical for central nervous system pathological conditions. The diagnostic value of several miRNAs was appraised in pediatric dysembryoplastic neuroepithelial tumors (DNETs) using miRNA microarrays and receiving operating characteristic curves analyses. Overall, five pediatric DNETs were studied. As controls, 17 samples were used: the FirstChoice Human Brain Reference RNA and 16 samples from deceased children who underwent autopsy and were not present with any brain malignancy. The miRNA extraction was carried out using the mirVANA miRNA Isolation Kit, while the experimental approach included miRNA microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction was performed to validate the expression profiles of miR-1909* and miR-3138 in all samples initially screened with miRNA microarrays. Our findings indicated that miR-3138 might act as a tumor suppressor gene when down-regulated and miR-1909* as a putative oncogenic molecule when up-regulated in pediatric DNETs compared to the control cohort. Subsequently, both miRNA signatures might serve as putative diagnostic biomarkers for pediatric DNETs.
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Author Contributions
MB conceived and designed the study, performed all experiments, evaluated and interpreted data analyses and drafted the manuscript. GIL performed all data analyses and participated in interpretation of data analyses. SAP performed resections of the postmortem specimens, KS performed tumor diagnosis, NP performed tumor resections and EK participated in the coordination and supervision of the study. All authors approved the final manuscript.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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Braoudaki, M., Lambrou, G.I., Papadodima, S.A. et al. MicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors. Med Oncol 33, 5 (2016). https://doi.org/10.1007/s12032-015-0719-3
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DOI: https://doi.org/10.1007/s12032-015-0719-3