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Medical Oncology

, 32:198 | Cite as

Expression of 3-phosphoinositide-dependent protein kinase 1 in colorectal cancer as a potential therapeutic target

  • Zhenglei Xu
  • Bihong Liao
  • Ru Zhang
  • Jun Yao
  • Ruiyue Shi
  • Lisheng WangEmail author
Original Paper

Abstract

3-Phosphoinositide-dependent protein kinase 1 (PDK1) is centrally involved in cancer progression, including proliferation, apoptosis and invasion. However, its expression pattern and possible cellular functions in human colorectal cancer remain unclear. In the present study, we show that PDK1 expression is up-regulated at both mRNA and protein levels in colorectal cancer clinical specimens and cell lines. Transient knockdown of PDK1 suppresses cellular growth, induces cellular apoptosis and causes abnormal cell cycle distribution. Meanwhile, decreased PDK1 level is closely associated with reduced Akt/cyclin D1 activity. Activating AKT activity and reintroducing cyclin D1 expression significantly compromised the oncogenic activity induced by PDK1. Together, our findings elucidate a key role for PDK1 in colorectal cellular functions trigged by the Akt/cyclin D1 pathway, thus providing a novel insight of PDK1 in colorectal carcinogenesis.

Keywords

PDK1 Colorectal cancer Cell proliferation Cell apoptosis Cell cycle 

Notes

Conflict of interest

The authors declare that there is no conflict of interests.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Zhenglei Xu
    • 1
  • Bihong Liao
    • 2
  • Ru Zhang
    • 1
  • Jun Yao
    • 1
  • Ruiyue Shi
    • 1
  • Lisheng Wang
    • 1
    Email author
  1. 1.Department of Gastroenterology, Shenzhen People’s HospitalSecond Clinical Medical College of Jinan UniversityShenzhenPeople’s Republic of China
  2. 2.Department of Cardiology, Shenzhen People’s HospitalSecond Clinical Medical College of Jinan UniversityShenzhenPeople’s Republic of China

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