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Medical Oncology

, 32:193 | Cite as

Crizotinib-loaded polymeric nanoparticles in lung cancer chemotherapy

  • Zhi-Ming Jiang
  • Shou-Ping Dai
  • Yong-Qing Xu
  • Tao Li
  • Jian Xie
  • Chong Li
  • Zhong-Hui ZhangEmail author
Original Paper

Abstract

The study describes the development of polylactide-tocopheryl polyethylene glycol 1000 succinate (PLA-TPGS)-based nanosystem as a carrier of crizotinib (CZT) to achieve superior anticancer efficacy in lung cancer therapy. We have demonstrated that block copolymer and hydrophobic drug is capable of self-assembling into a very stable nanocarrier, with suitable properties that allow their application for cancer drug delivery. Drug release study showed a sustained release pattern as a result of entrapment in the hydrophobic core of micelles. CZT/PT NP showed a noticeable cytotoxic effect in NCIH3122 lung cancer cells in a dose-dependent manner. Furthermore, morphological imaging and Live/Dead assay revealed a superior anticancer efficacy for nanoformulations. The polymeric nanoparticle showed a predominant presence in the cytoplasmic region of cell, indicating a typical endocytosis-mediated cellular uptake. The annexin V/PI staining-based apoptosis assay showed a remarkable ~40 % apoptosis (early and late apoptosis cells) comparing to only ~25 % apoptosis by free CZT. Taken together, Vitamin E TPGS-modified PLA nanoparticles would be a potential drug delivery system to increase the chemotherapeutic efficacy of CZT in lung cancer chemotherapy.

Keywords

Lung cancer Crizotinib Apoptosis Polymeric nanoparticles Cell viability 

Notes

Acknowledgments

The study was supported from the research grant of Shandong University. Authors appreciate the help of Dr. Wei Fan for proof-reading the entire manuscript.

Conflict of interest

The authors report no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Zhi-Ming Jiang
    • 1
  • Shou-Ping Dai
    • 2
  • Yong-Qing Xu
    • 1
  • Tao Li
    • 1
  • Jian Xie
    • 1
  • Chong Li
    • 1
  • Zhong-Hui Zhang
    • 1
    Email author
  1. 1.Department of Critical-Care MedicineQianfoshan Hospital Affiliated to Shandong UniversityJinanChina
  2. 2.Department of ImagingLinyi People’s Hospital Affiliated to Shandong UniversityLinyiChina

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