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Medical Oncology

, 32:194 | Cite as

SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway

  • Yao Lu
  • Bin Hu
  • Guo-Feng Guan
  • Jie Chen
  • Chun-qiu Wang
  • Qiong Ma
  • Yan-Hua Wen
  • Xiu-Chun Qiu
  • Xiao-ping ZhangEmail author
  • Yong ZhouEmail author
Original Paper

Abstract

Osteosarcoma (OS), the most common primary malignant bone tumor in children and adolescents, lacks an effective therapy. Stromal cell-derived factor (SDF-1) and its receptor, CXCR4, play multiple roles in migration, proliferation, and survival of different tumor cells. This study aimed to investigate whether the functional SDF-1/CXCR4 signaling mediates chemotaxis in F5M2 OS cells as well as the underlying mechanisms. Immunohistochemistry and immunofluorescence microscopy were used. RNA expression was detected by real-time quantitative polymerase chain reaction, and protein expression was examined by Western blotting. Migration assays were carried out in F5M2 cells. The results showed that the expression of CXCR4 and β-catenin mRNA and protein was significantly higher in OS tissues compared to the surrounding non-neoplastic tissues. SDF-1 promoted F5M2 cell migration by activating the AKT and Wnt/β-catenin signaling pathway, which was abrogated by preincubation with AMD3100 and LY294002. In conclusion, SDF-1/CXCR4 axis-promoted F5M2 cell migration was regulated by the Wnt/β-catenin signaling pathway.

Keywords

Osteosarcoma SDF-1 CXCR4 Wnt/β-catenin Metastasis 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (Nos. 81272441, 81201633, 81372297).

Conflict of interest

The authors declare that there is no conflict of interest in this study.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yao Lu
    • 1
  • Bin Hu
    • 2
  • Guo-Feng Guan
    • 1
  • Jie Chen
    • 1
  • Chun-qiu Wang
    • 3
  • Qiong Ma
    • 1
  • Yan-Hua Wen
    • 1
  • Xiu-Chun Qiu
    • 1
  • Xiao-ping Zhang
    • 1
    Email author
  • Yong Zhou
    • 1
    Email author
  1. 1.Department of Orthopaedic Surgery, Tangdu HospitalThe Fourth Military Medical UniversityXi’anChina
  2. 2.Department of Haematology, Tangdu HospitalThe Fourth Military Medical UniversityXi’anChina
  3. 3.Department of Orthopedic Surgery359 Hospital of Chinese PLAZhenjiangChina

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