Abstract
In recent decades, the CXC chemokine receptor 7 (CXCR7) and its ligand CCL21 have been extensively reported to be associated with tumorigenesis. Meanwhile, Slug signaling induces the epithelial-mesenchymal transition (EMT) process in chondrosarcoma development. In the present study, we explored the functions of CCL21/CXCR7 in Slug-mediated EMT in the chondrosarcoma. We analyzed protein expression of CXCR7 and Slug in human chondrosarcoma samples. Effects of CXCR7 on chondrosarcoma cells were assessed by in vitro assays. Additionally, CXCR7 pathways were further investigated by pharmacological and genetic approaches. We found that the altered CXCR7 (81.7 %) and Slug (85.0 %) expression in human chondrosarcoma tissues were significantly associated with grade, recurrence, and 5-year overall survival. According to in vitro assays, CCL21 stimulation induced the expression of phosph-ERK, phosph-AKT, Slug and N-cadherin in SW1353 cells, while the expression of E-cadherin was down-regulated. Furthermore, Slug signaling modulated E- to N-cadherin switch, which was influenced by the kinase inhibitor PD98059 and LY294002. In addition, the genetic silencing of Slug inhibited the capacity of migration and invasion of SW1353 cells. In conclusion, CCL21/CXCR7 pathway activates ERK and PI3K/AKT signallings to up-regulate Slug pathway, leading to the occurrence of EMT process in human chondrosarcoma. This study lays a new foundation for molecule-targeted therapy of human chondrosarcoma.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12032-014-0478-6/MediaObjects/12032_2014_478_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12032-014-0478-6/MediaObjects/12032_2014_478_Fig2_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12032-014-0478-6/MediaObjects/12032_2014_478_Fig3_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12032-014-0478-6/MediaObjects/12032_2014_478_Fig4_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12032-014-0478-6/MediaObjects/12032_2014_478_Fig5_HTML.gif)
Similar content being viewed by others
References
Randall RL, Bruckner J, Lloyd C, et al. Sacral resection and reconstruction for tumors and tumor-like conditions. Orthopedics. 2005;28:307–13.
Bergh P, Gunterberg B, Meis-Kindblom JM, et al. Prognostic factors and outcome of pelvic, sacral, and spinal chondrosarcomas: a center-based study of 69 cases. Cancer. 2001;91:1201–12.
Bovée JV, Cleton-Jansen AM, Taminiau AH, et al. Emerging pathways in the development of chondrosarcoma of bone and implications for targeted treatment. Lancet Oncol. 2005;6:599–607.
Tan ML, Shao P, Friedhuber AM, et al. The potential role of free chitosan in bone trauma and bone cancer management. Biomaterials. 2014;35:7828–38.
Uchida A, Seto M, Hashimoto N, et al. Molecular diagnosis and gene therapy in musculoskeletal tumors. J Orthop Sci. 2000;5:418–23.
Gelderblom H, Hogendoorn PC, Dijkstra SD, et al. The clinical approach towards chondrosarcoma. Oncologist. 2008;13:320–9.
Ishikawa T, Shimizu T, Ueki A, et al. Twist2 functions as a tumor suppressor in murine osteosarcoma cells. Cancer Sci. 2013;104:880–8.
Casas E, Kim J, Bendesky A, et al. Snail2 is an essential mediator of Twist1-induced epithelial mesenchymal transition and metastasis. Cancer Res. 2011;71:245–54.
Gonzalez DM, Medici D. Signaling mechanisms of the epithelial-mesenchymal transition. Sci Signal. 2014;7:re8.
Tirino V, Camerlingo R, Bifulco K, et al. TGF-beta1 exposure induces epithelial to mesenchymal transition both in CSCs and non-CSCs of the A549 cell line, leading to an increase of migration ability in the CD133+ A549 cell fraction. Cell Death Dis. 2013;4:e620.
Eberlein C, Rooney C, Ross SJ, et al. E-cadherin and EpCAM expression by NSCLC tumour cells associate with normal fibroblast activation through a pathway initiated by integrin alphavbeta6 and maintained through TGF beta signalling. Oncogene. 2014. doi:10.1038/onc.2013.600.
Liu RY, Zeng Y, Lei Z, et al. JAK/STAT3 signaling is required for TGF-beta-induced epithelial-mesenchymal transition in lung cancer cells. Int J Oncol. 2014;44:1643–51.
Jing Y, Han Z, Zhang S, et al. Epithelial-mesenchymal transition in tumor microenvironment. Cell Biosci. 2011;1:29.
Zheng H, Kang Y. Multilayer control of the EMT master regulators. Oncogene. 2014;33:1755–63.
Nieto MA, Sargent MG, Wilkinson DG, et al. Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science. 1994;264:835–59.
Haupt S, Alsheich-Bartok O, Haupt Y. Clues from worms: a Slug at Puma promotes the survival of blood progenitors. Cell Death Differ. 2006;13:913–5.
Umar S. Enteric pathogens and cellular transformation: bridging the gaps. Oncotarget. 2014;5:6573–5.
Ding W, You H, Dang H, et al. Epithelial-to-mesenchymal transition of murine liver tumor cells promotes invasion. Hepatology. 2010;52:945–53.
Cicchini C, Laudadio I, Citarella F, et al. TGFbeta-induced EMT requires focal adhesion kinase (FAK) signaling. Exp Cell Res. 2008;314:143–52.
Leckband D. Beyond structure: mechanism and dynamics of intercellular adhesion. Biochem Soc Trans. 2008;36:213–20.
Hsu YL, Hou MF, Kuo PL, et al. Breast tumor-associated osteoblast-derived CXCL5 increases cancer progression by ERK/MSK1/Elk-1/snail signaling pathway. Oncogene. 2013;32:4436–47.
Li Y, Jia L, Ren D, et al. Axl mediates tumor invasion and chemosensitivity through PI3K/Akt signaling pathway and is transcriptionally regulated by slug in breast carcinoma. IUBMB Life. 2014;66:507–18.
Suman S, Kurisetty V, Das TP, et al. Activation of AKT signaling promotes epithelial-mesenchymal transition and tumor growth in colorectal cancer cells. Mol Carcinog. 2014;53:E151–60.
Li Y, Zhao Z, Xu C, et al. HMGA2 induces transcription factor Slug expression to promote epithelial-to-mesenchymal transition and contributes to colon cancer progression. Cancer Lett. 2014;355:130–40.
He LC, Gao FH, Xu HZ, et al. Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells. Oncol Rep. 2012;28:1399–405.
Acknowledgments
We gratefully thank Mingtang He and Ziqing Zhang for their critical reading of this paper and valuable suggestions.
Conflict of interest
None.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, G., Yang, Y., Xu, S. et al. Slug signaling is up-regulated by CCL21/CXCR7 to induce EMT in human chondrosarcoma. Med Oncol 32, 2 (2015). https://doi.org/10.1007/s12032-014-0478-6
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s12032-014-0478-6