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Melanoma-associated antigen genes: a new trend to predict the prognosis of breast cancer patients

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Abstract

MAGE-A are normally expressed in testis and placenta. Among MAGEs, the MAGE-A subtype has been the most characterized in cancers. Our study was conducted to assess the expression of (MAGE-A1–MAGE-A6) m-RNA using MMRPs and MAGE-A12 m-RNA in blood for evaluating their clinical implications in breast cancer patients. RT-PCR was carried out to detect the expression of (MAGE-A1–MAGE-A6) m-RNA using MMRPs and MAGE-A12 m-RNA in blood. The study included 100 breast cancer cases aged 41–62 years and 100 controls aged 36–53 years. MAGE m-RNA expression was not detected in healthy donors. In breast cancer patients, the positivity of (MAGE-A1–MAGE-A6) m-RNA was 44 % (44 cases), while MAGE-A12 m-RNA was expressed in 13 % (13 cases). The gene expressions of MAGE-A1–A6 and MAGE-A12 were significantly associated with advanced TNM stages (P = 0.001 and 0.034, respectively). Simultaneous estimation of the gene expressions of MAGE-A1–A6 and MAGE-A12 can detect occult hematogenous dissemination of tumor cells and may help to monitor the effectiveness of the therapy and the development of effective immunotherapeutic strategies in breast cancer.

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Abbreviations

CTC:

Circulating tumor cells

MAGE:

Melanoma-associated antigen gene

RT-PCR:

Reverse transcription-polymerase chain reaction

MMRPs:

Multiple MAGE-recognizing primers

FNAC:

Fine needle aspiration cytology

PBMCs:

Peripheral blood mononuclear cells

DEPC:

Diethylpyrocarbonate

CEA:

Carcinoembryonic antigen

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Authors and Affiliations

  1. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Eman Abd-Elkader Abd-Elsalam

  2. General Surgery Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Nadia A. Ismaeil

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  1. Eman Abd-Elkader Abd-Elsalam
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  2. Nadia A. Ismaeil
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Correspondence to Eman Abd-Elkader Abd-Elsalam.

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Abd-Elsalam, E.AE., Ismaeil, N.A. Melanoma-associated antigen genes: a new trend to predict the prognosis of breast cancer patients. Med Oncol 31, 285 (2014). https://doi.org/10.1007/s12032-014-0285-0

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