The preliminary study of p53 codon 72 polymorphism and risk of cervical carcinoma in Gabonese women
- 206 Downloads
Cervical cancer is the leading cause of cancer-related death in Africa and the first most common cancer in Gabonese women due to infection of high-risk human papillomavirus (HPV). However, other cofactors such as genetic factors also come into play. A common polymorphism of the p53 codon 72 in exon 4 with two alleles encoding arginine or proline is known at this locus. The homozygous arginine form of this polymorphism has been associated with the development of cervical cancer as an increased genetic risk factor. However, the results are still controversial. This study aims to investigate whether the genotype distribution of p53 codon 72 may be a risk factor for cervical cancer among Gabonese women. Samples from 102 Gabonese women, 31 diagnosed with cervical cancer and 71 healthy controls, were used. HPV detection was done by nested PCR with MY09/11 and GP5+/6+ primers followed by sequencing for HPV genotyping. p53 codon 72 polymorphism determination was performed by allele-specific PCR assay. Viral DNA was detected in 87.1 % of cases and in 54.93 % of control. HPV 16 was the most predominant in cancer and controls cases. The distribution of Arg/Arg, Arg/Pro and Pro/Pro genotypes was 35.5, 51.6 and 12.9 % in the cervical cancer group and 22.5, 62 and 15.5 % in the control group. No significant association was found between polymorphism of p53 itself as well as in combination with HPV16/18 infection and risk of development of cervical cancer among Gabonese women. Thus, the polymorphism of p53 codon 72 in exon 4 does not seem to play a role in the development of cervical cancer among Gabonese women.
Keywordsp53 codon 72 Polymorphism Cervical cancer Human papillomavirus Gabonese women
The authors would like to express gratitude to Dr Mabicka Mabicka Barthélemy, Head of “Département d’Anatomie et de Cytologie Pathologiques de l’Université des Sciences de la Santé de Libreville,” Sophia Abassi and all technical staff of “Département d’Anatomie et de Cytologie Pathologiques de l’Université des Sciences de la Santé de Libreville.” We would also like to thank Pr. Ernerst Belembaogo, Head of “Institut de cancérologie d’Agondjé” and his staff for their precious technical assistance. This project was financially supported by the Moroccan Minister of Higher Education. We have also received technical assistance from UATRS-CNRST, Morocco for genotyping.
Conflict of interest
- 2.Li N, Franceschi S, Howell-Jones R, Snijders PJ, Clifford GM. Human papillomavirus type distribution in 30,848 invasive cervical cancers worldwide: variation by geographical region, histological type and year of publication. Int J Cancer J Int Du Cancer. 2011;128(4):927–35. doi: 10.1002/ijc.25396.CrossRefGoogle Scholar
- 13.Khair MM, Mzibri ME, Mhand RA, Benider A, Benchekroun N, Fahime EM, et al. Molecular detection and genotyping of human papillomavirus in cervical carcinoma biopsies in an area of high incidence of cancer from Moroccan women. J Med Virol. 2009;81(4):678–84. doi: 10.1002/jmv.21279.PubMedCrossRefGoogle Scholar
- 23.Tenti P, Vesentini N, Rondo Spaudo M, Zappatore R, Migliora P, Carnevali L, et al. p53 codon 72 polymorphism does not affect the risk of cervical cancer in patients from northern Italy. Cancer epidemiol biomarkers prev. 2009;9(4):435–8.Google Scholar
- 26.El khair MM, Ennaji MM, El kebbaj R, Mhand RA, Attaleb M, El Mzibri M. p53 codon 72 polymorphism and risk of cervical carcinoma in Moroccan women. Med Oncol. 2010; 27(3):861–6. doi: 10.1007/s12032-009-9297-6
- 33.BrennaI SMF dSI, Zeferino LC, Pereira J, Martinez EZ, Syrjänen KJ Prevalence of codon 72 P53 polymorphism in Brazilian women with cervix cancer. Genet Mol Biol. 2004; 27(4). doi: 10.1590/S1415-47572004000400005