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Overexpression of miR-223 correlates with tumor metastasis and poor prognosis in patients with colorectal cancer

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Abstract

The purpose of the study was to investigate microRNA-223 (miR-223) expression in colorectal cancer (CRC) and its relationship with tumorigenesis and disease prognosis. Quantitative real-time PCR was used to measure levels of miR-223 in tumor samples and adjacent non-cancerous tissues from 62 patients undergoing radical resection for the treatment of CRC. The associations between miR-223 expression and patient age, sex, as well as clinicopathologic parameters, such as tumor size, differentiation, location, invasion depth, metastasis, tumor-node-metastasis (TNM) stage, and overall patient survival, were analyzed by Mann–Whitney U and Kruskal–Wallis tests. Kaplan–Meier method and Cox proportional hazards regression analyses were performed to estimate the prognostic factors for patient survival prediction. The expression of miR-223 was significantly upregulated in CRC tissues compared with adjacent non-cancerous tissues (P < 0.05). This overexpression was associated with TNM stage and lymph node and distant metastases, (P < 0.05). Moreover, Kaplan–Meier analysis demonstrated that patients with high miR-223 expression had a poorer overall survival (OS) than those with low miR-223 expression (P = 0.002). Univariate analysis revealed a statistically significant correlation between OS and miR-223 level, histology grade, metastasis and TNM stage (P < 0.001). Furthermore, miR-223 levels and histology grade were independently associated with OS (HR 0.204, 95 % CI 0.101–0.415, P < 0.05 and HR 2.252, 95 % CI 1.429–3.546, P < 0.05, respectively). The overexpression of miR-223 may play an important role in the progression of CRC and can be used as an independent factor to determine CRC prognosis.

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Acknowledgments

Our work was supported by National Key Clinical Medical Specialties Foundation and National Natural Science Foundation of China (81271916, 81301506).

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The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Clinical Laboratory, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China

    Ze-wu Li, Yong-mei Yang, Lu-tao Du, Zhaogang Dong, Li-li Wang, Xin Zhang, Xuan-jun Zhou, Gui-xi Zheng, Ai-lin Qu & Chuan-xin Wang

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  1. Ze-wu Li
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  2. Yong-mei Yang
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  3. Lu-tao Du
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  4. Zhaogang Dong
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  5. Li-li Wang
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  6. Xin Zhang
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  7. Xuan-jun Zhou
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  8. Gui-xi Zheng
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  9. Ai-lin Qu
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  10. Chuan-xin Wang
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Correspondence to Chuan-xin Wang.

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Li, Zw., Yang, Ym., Du, Lt. et al. Overexpression of miR-223 correlates with tumor metastasis and poor prognosis in patients with colorectal cancer. Med Oncol 31, 256 (2014). https://doi.org/10.1007/s12032-014-0256-5

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  • DOI: https://doi.org/10.1007/s12032-014-0256-5

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