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Knocking down the expression of SYF2 inhibits the proliferation of glioma cells

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Abstract

SYF2 is thought to be a cell cycle regulator at the G1/S transition, which encodes a nuclear protein that interacts with cyclin D-type binding-protein 1. In the present study, we investigated the role of SYF2 in human glioma progression. Immunohistochemical and Western blot analyses were performed in human glioma tissues. High SYF2 expression (located in cell nuclei) was observed in 80 samples, and its level was correlated with the grade of malignancy. A strongly positive correlation was observed between SYF2 and Ki-67 expression (P < 0.01). More importantly, high expression of SYF2 was associated with a poor outcome. In vitro, after the release of U87 cell lines from serum starvation, the expression of SYF2 was upregulated, as well as PCNA and cyclin D1. In addition, knockdown of SYF2 by small interfering RNA transfection diminished the expression of PCNA, cyclin D1 and arrested cell growth at G1 phase. These results indicate that SYF2 in glioma is essential for cell proliferation; thus, targeting SYF2 or its downstream targets may lead to novel therapies for glioblastomas.

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Acknowledgments

This work was supported by the China Natural Science Foundation (81201976, 81000963), Jiangsu Province’s Natural Science Foundation (BK2012670), Jiangsu Province’s Health Department (z201318), Yancheng Medical Science Development Foundation (YK2013003, YK2013019).

Conflict of interest

All authors have declared all sources of funding for the research reported in this manuscript and have no financial or other contractual agreements that might cause conflicts of interest or be perceived as causing conflicts of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, The First People’s Hospital of Yancheng Affiliated with Nantong University, Yancheng, 224001, People’s Republic of China

    Jun Guo & Guan Sun

  2. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu Province, People’s Republic of China

    Lixiang Yang

  3. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, People’s Republic of China

    Jianfeng Huang, Xiancheng Liu, Xiaojun Qiu, Tao Tao, Yonghua Liu, Xiaojuan He, Na Ban & Shaochen Fan

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  1. Jun Guo
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  11. Guan Sun
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Correspondence to Guan Sun.

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Jun Guo and Lixiang Yang have contributed equally in this work.

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12032_2014_101_MOESM1_ESM.tif

Fig. S1 The negative control of immunohistochemical analyses. Negative control slides were processed in parallel using a nonspecific immunoglobulin IgG at the same concentration as the primary antibody

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Guo, J., Yang, L., Huang, J. et al. Knocking down the expression of SYF2 inhibits the proliferation of glioma cells. Med Oncol 31, 101 (2014). https://doi.org/10.1007/s12032-014-0101-x

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