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Medical Oncology

, 31:52 | Cite as

Upregulation HOXA10 homeobox gene in endometrial cancer: role in cell cycle regulation

  • Lin Zhang
  • Yicong Wan
  • Yi Jiang
  • Jingjing Ma
  • Jinsong Liu
  • Weiwei Tang
  • Xinru Wang
  • Wenjun ChengEmail author
Original Paper

Abstract

Homeobox genes encode a group of transcription factors that regulate embryonic development, organ differentiation and cell proliferation. Some researchers found that homeobox gene family could regulate the expression of cell cycle-related factors which are involved in tumorigenesis. The aim of the present study was to determine whether HOXA10 gene could regulate the expression of p21 and control the cell cycle in endometrial cancer. Real-time PCR and Western blot were used to analyze HOXA10 and p21 gene expression in tissue samples of normal endometrium and endometrial cancer. Upregulate and downregulate HOXA10 gene in endometrial cancer cells by HOXA10-vector and HOXA10-siRNA, p21 mRNA and protein expression were tested by real-time PCR and Western blot. FCM analyze the cell cycle alteration, and cell count and EdU assay were recruited to detect cell proliferative activity after upregulation of HOXA10. Expression of HOXA10 and p21 gene decreased in endometrial cancer, and the expression level of HOXA10 was correlated with cancer grade. The percentage of G1 phase cells increased, and cell proliferative activity decreased after upregulate HOXA10 expression. The expression of p16, p27, Myc, Cyclin D1, Cyclin E, CDK2, CDK4 and CDK6 did not alter after upregulation HOXA10. P21 gene mRNA and protein increased/decreased after upregulation/downregulation HOXA10 expression in cancer cells. HOXA10 regulates G1 phase arrest in endometrial cancer which may be mediated by p21.

Keywords

Endometrial cancer Homeobox gene A10 p21 

Abbreviations

HOXA10

Homeobox gene A10

p21

p21waf1/cip1 gene

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30772332), (81272871), Nature Science Foundation of the Science and Technology Department of Jiangsu Province (BK2010576), Jiangsu Province Six Summit Talent Foundation (303070774IB09) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD].

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

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Supplementary material 1 (JPEG 41 kb)
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Supplementary material 2 (JPEG 162 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Lin Zhang
    • 1
  • Yicong Wan
    • 1
  • Yi Jiang
    • 1
  • Jingjing Ma
    • 1
  • Jinsong Liu
    • 2
  • Weiwei Tang
    • 1
  • Xinru Wang
    • 3
  • Wenjun Cheng
    • 1
    • 3
    Email author
  1. 1.The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina
  2. 2.Pathology and Cancer BiologyUniversity of Texas M.D. Anderson Cancer CenterHoustonUSA
  3. 3.State Key Laboratory of Reproductive Medicine, Institution of ToxicologyNanjing Medical UniversityNanjingChina

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