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Overexpression of RPS6KB1 predicts worse prognosis in primary HCC patients

Medical Oncology volume 29, pages 3070–3076 (2012)Cite this article

Abstract

RPS6KB1 (ribosomal protein S6 kinase, 70 kDa, polypeptide 1) plays a key role in regulating protein translation. The role of RPS6KB1 in HCC (hepatocellular carcinoma) has not been fully investigated. This study was undertaken to determine the clinicopathological features and prognostic value of RPS6KB1 in HCC. We examined RPS6KB1 expression in 30 paired liver cancer tissues and adjacent noncancerous tissues by reverse transcription real-time PCR and Western blotting. In addition, we analyzed RPS6KB1 expression in 87 HCC samples by immunohistochemistry. The expression of RPS6KB1 was significantly increased in cancer tissues. Clinicopathological analysis showed that the expression of RPS6KB1 was significantly correlated with tumor size, histopathologic classifications, and serum alpha-fetoprotein (AFP). Kaplan–Meier survival curves revealed that increased expression of RPS6KB1 correlated with poor prognosis of HCC patients. RPS6KB1 expression was an independent prognostic marker for overall survival of HCC patients by multivariate analysis. Our data suggest that RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81171891).

Conflict of interest

The authors have declared that no conflict of interest exists.

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Affiliations

  1. State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangdong, 510060, China

    Pin Dong Li, Mei Ying Zhang, Lin Jing Yuan, Yin Lian Cha, Xiao Fang Ying & Hui Yun Wang

  2. Cancer Center of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430023, Hubei, China

    Pin Dong Li & Gang Wu

  3. Cancer Genetics Program, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China

    Wen Jie Zhang

Authors
  1. Pin Dong Li
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  2. Wen Jie Zhang
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  3. Mei Ying Zhang
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  4. Lin Jing Yuan
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  5. Yin Lian Cha
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  6. Xiao Fang Ying
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  7. Gang Wu
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  8. Hui Yun Wang
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Corresponding author

Correspondence to Hui Yun Wang.

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This work was performed in Sun Yat-sen University Cancer Center.

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Li, P.D., Zhang, W.J., Zhang, M.Y. et al. Overexpression of RPS6KB1 predicts worse prognosis in primary HCC patients. Med Oncol 29, 3070–3076 (2012). https://doi.org/10.1007/s12032-012-0268-y

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  • DOI: https://doi.org/10.1007/s12032-012-0268-y

Keywords

  • RPS6KB1
  • Hepatocellular carcinoma
  • Prognosis
  • Western blotting
  • Immunohistochemistry