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The relationship of the active and latent forms of TGF-β1 with marrow fibrosis in essential thrombocythemia and primary myelofibrosis

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Abstract

The aim of this study was to perform an immunohistochemical analysis from 100 megakaryocytes per sample, analyzing positivity and intensity levels of anti-LAP human TGF-β1 (or Latent TGF-β1) and anti-TGF-β1 (or Active TGF-β1) antibodies from 18 essential thrombocythemia (ET) and 38 primary myelofibrosis (PMF) patients (being 19 pre-fibrotic and 19 fibrotic). Six bone marrow donor biopsies were used as controls. Fibrosis in bone marrow biopsies (BMB) was evaluated according to the European Consensus. The average fibrosis grade differed between each group (P = 0.001 or P = 0.003). Latent TGF-β1 values differed significantly between pre-fibrotic (P = 0.018) and fibrotic (P = 0.031) groups when compared with the control group. The high immunoexpression level of Latent TGF-β1 in the megakaryocytes from patients with myelofibrosis, which was not observed in patients with essential thrombocythemia, may be associated with the development of bone marrow fibrosis.

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Acknowledgments

The authors are grateful to Antonio Carlos de Sousa for his excellent technical assistance and to Fabio Tadeu Montesano (Universidade Federal de São Paulo) for statistical analyses. This project was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior) and partially supported by the FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) grant 07-55948-8.

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Authors and Affiliations

  1. Department of Pathology, Universidade Federal de São Paulo, Vicente de Carvalho Avenue, 65, # 72, São Paulo, Santos, 11045-501, Brazil

    Cesar Cilento Ponce, Silvia Saiuli M. Ihara & Maria Regina R. Silva

  2. Department of Hematology, Universidade Federal de São Paulo, São Paulo, Brazil

    Maria de Lourdes F. Chauffaille

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  1. Cesar Cilento Ponce
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  2. Maria de Lourdes F. Chauffaille
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  3. Silvia Saiuli M. Ihara
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  4. Maria Regina R. Silva
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Correspondence to Cesar Cilento Ponce.

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Ponce, C.C., de Lourdes F. Chauffaille, M., Ihara, S.S.M. et al. The relationship of the active and latent forms of TGF-β1 with marrow fibrosis in essential thrombocythemia and primary myelofibrosis. Med Oncol 29, 2337–2344 (2012). https://doi.org/10.1007/s12032-011-0144-1

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  • DOI: https://doi.org/10.1007/s12032-011-0144-1

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