Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells
- 425 Downloads
Cancer stem cells (CSCs) play a critical role in both cancer initiation and relapse as they are resistant to most cytotoxic agents and able to proliferate indefinitely. The plant alkaloid oxymatrine has many biological activities including the ability to induce cell cycle arrest and apoptosis, which makes it a potentially useful agent for targeting cancer cells. In order to determine whether it has beneficial pharmacological properties to eradicate CSCs, we analyzed the effects of oxymatrine on MCF-7 breast cancer cells. Cancer stem-like cells’ (side population, SP) identification and sorting were performed. The inhibitory effect of oxymatrine was evaluated on the sorted SP and non-SP cells. The results indicated that oxymatrine caused a dose-dependent reduction in the proliferation of MCF-7 cells and a decrease in SP cells. Wnt/β-catenin signaling pathway was also examined by analyzing the expression of total β-catenin and phosphorylated β-catenin in cytoplasm, and the results showed that the growth inhibitory effects of oxymatrine treatment on MCF-7 cells may be due to the inhibition of SP and Wnt/β-catenin signaling pathway. Further work is warranted to explore whether oxymatrine may be a useful novel therapeutic drug for targeting breast CSCs.
KeywordsOxymatrine MCF-7 Breast cancer Cancer stem cell Side population β-Catenin
- 1.Zhou S, Schuetz JD, Bunting KD, Colapietro AM, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, Sorrentino BP. The abc transporter bcrp1/abcg2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001;7:1028–34.PubMedCrossRefGoogle Scholar
- 19.Chui CH, Lau FY, Tang JC, Kan KL, Cheng GY, Wong RS, Kok SH, Lai PB, Ho R, Gambari R, Chan AS. Activities of fresh juice of scutellaria barbata and warmed water extract of radix sophorae tonkinensis on anti-proliferation and apoptosis of human cancer cell lines. Int J Mol Med. 2005;16:337–41.PubMedGoogle Scholar
- 21.Loontiens FG, Regenfuss P, Zechel A, Dumortier L, Clegg RM. Binding characteristics of hoechst 33258 with calf thymus DNA, poly[d(a-t)], and d(ccggaattccgg): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry. 1990;29:9029–39.PubMedCrossRefGoogle Scholar