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Medical Oncology

, Volume 28, Issue 4, pp 986–990 | Cite as

{2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-d-Glucose} suppresses proliferation and induces apoptosis in the human esophageal cancer cell line

  • Jing WuEmail author
  • Hong Lu
  • Xiangchun Ling
  • Canghai Wang
  • Ji Rui
  • Aiqin Wang
  • Liang Qiao
Original Paper
  • 120 Downloads

Abstract

The aim of this study was to investigate the molecular mechanisms of apoptosis induced by {2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-d-Glucose} (COPADG) in the esophageal cancer cell line Eca-109 and to establish a relationship between the rate of apoptosis and Fas and Bcl-2 protein expression. Eca-109 cells were cultured under standard condition. Cell growth was measured by MTT assay. Apoptosis and cell proliferation were determined by flow cytometry. Expressions of apoptosis-regulated genes Fas and Bcl-2 were detected by immunohistochemical methods and image analysis. COPADG dose- and time-dependently inhibited the growth of Eca-109 cells in vitro. Incubation of Eca-109 cells with 40 μmol/l of COPADG for 48 h induced significant apoptosis. After drug treatment, Fas protein expression was increased, while Bcl-2 protein expression was decreased. COPADG is able to induce apoptosis in the esophageal cancer cell line Eca-109. The mechanism of apoptosis in these cells may be related to up-regulation of Fas gene expression and the down-regulation of Bcl-2, which may serve as the experimental evidence for development of new drugs for the non-surgical management of human esophageal cancer.

Keywords

Esophageal cancer Apoptosis {2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-D-Glucose} Fas Bcl-2 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jing Wu
    • 1
    • 2
    Email author
  • Hong Lu
    • 2
  • Xiangchun Ling
    • 1
  • Canghai Wang
    • 1
  • Ji Rui
    • 2
  • Aiqin Wang
    • 3
  • Liang Qiao
    • 2
    • 4
  1. 1.Department of Gastroenterology and HepatologyBeijing Shijitan HospitalBeijingChina
  2. 2.Department of Gastroenterology and HepatologyFirst Teaching Hospital of Lanzhou UniversityLanzhouChina
  3. 3.Lanzhou Institute of Chemical Physics, Chinese Academy of SciencesLanzhouChina
  4. 4.Department of Gastroenterology and Hepatology, Storr Liver Unit, Westmead Millennium InstituteUniversity of Sydney at Westmead HospitalWestmeadAustralia

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