Abstract
MicroRNAs (miRNAs) are non-coding RNAs that regulate the expression of target mRNAs. Altered expression of specific miRNAs in several tumor types has been reported. However, the expression levels of miR-31 in gastric cancers are unclear. The objective of the present study was to compare the expression profile of miR-31 between gastric cancer tissues and non-tumor tissues. Real-time quantitative reverse transcription-polymerase chain reaction technology was used to detect the levels of miR-31 expression. The expression levels of miR-31 in gastric cancer tissues were significantly lower than those in non-tumor tissues. This new information may help to clarify the molecular mechanisms involved in gastric carcinogenesis and to indicate that miR-31 may be a novel diagnostic biomarker of gastric cancer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Pisani P, Parkin DM, Bray F, Ferlay J. Estimates of the worldwide mortality from 25 cancers in 1990. Int J Cancer. 1999;83:18–29.
Murray CJ, Lopez AD. Alternative projections of mortality and disability by cause 1990–2020: global burden of disease study. Lancet. 1997;349:1498–504.
Gaspar MJ, Arribas I, Coca MC, Diez-Alonso M. Prognostic value of carcinoembryonic antigen, CA 19-9 and CA 72-4 in gastric carcinoma. Tumour Biol. 2001;22:318–22.
Marrelli D, et al. Prognostic significance of CEA, CA 19-9 and CA 72-4 preoperative serum levels in gastric carcinoma. Oncology. 1999;57:55–62.
Carpelan-Holmstrom M, Louhimo J, Stenman UH, Alfthan H, Haglund C. CEA, CA 19-9 and CA 72-4 improve the diagnostic accuracy in gastrointestinal cancers. Anticancer Res. 2002;22:2311–6.
Takahashi Y, et al. The usefulness of CEA and/or CA19-9 in monitoring for recurrence in gastric cancer patients: a prospective clinical study. Gastric Cancer. 2003;6:142–5.
Hatfield S, Ruohola-Baker H. microRNA and stem cell function. Cell Tissue Res. 2008;331:57–66.
Calin GA, Croce CM. MicroRNA-cancer connection: ‘the beginning of a new tale’. Cancer Res. 2006;66:7390–4.
Volinia S, et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 2006;103:2257–61.
Bandrés E, et al. Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues. Mol Cancer. 2006;5:29.
Chan SH, Wu CW, Li AF, Chi CW, Lin WC. miR-21 microRNA expression in human gastric carcinomas and its clinical association. Anticancer Res. 2008;28:907–11.
Zhang HH, Wang XJ, Li GX, Yang E, Yang NM. Detection of let-7a microRNA by real-time PCR in gastric carcinoma. World J Gastroenterol. 2007;13:2883–8.
Ji Q, et al. Restoration of tumor suppressor miR-34 inhibits human p53-mutant gastric cancer tumorspheres. BMC Cancer. 2008;8:266.
Guo J, et al. Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues. J Gastroenterol Hepatol. 2009;24:652–7.
Sobin LH, Wittekind CH, editors. TNM classification of malignant tumors 5th edition, International Union Against Cancer (UICC). New York: Wiley; 1997. p. 59–62.
Solcia E, et al. Endocrine tumours of the gastrointestinal tract. In: Solcia E, Klöppel G, Sobin LH, editors. Histological typing of endocrine tumours. 2nd ed. Berlin: Springer-Verlag; 2000. p. 61–8.
Lawrie CH, et al. Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 2008;141:672–5.
Peng JJ, et al. Predicting prognosis of rectal cancer patients with total mesorectal excision using molecular markers. World J Gastroenterol. 2007;13:3009–15.
Wang SL, et al. Increased expression of hLRH-1 in human gastric cancer and its implication in tumorigenesis. Mol Cell Biochem. 2008;308:93–100.
Yan LX, et al. MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis. RNA. 2008;14:2348–60.
Shen J, et al. MicroRNAs regulate ocular neovascularization. Mol Ther. 2008;16:1208–16.
Guglielmelli P, et al. MicroRNA expression profile in granulocytes from primary myelofibrosis patients. Exp Hematol. 2007;35:1708–18.
Wong QW, et al. MicroRNA-223 is commonly repressed in hepatocellular carcinoma and potentiates expression of Stathmin1. Gastroenterology. 2008;135:257–69.
Wong TS, et al. Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clin Cancer Res. 2008;14:2588–92.
Acknowledgments
This work was supported by Scientific Research Fund of Zhejiang Provincial Education Department (No. 20070965), Student Innovation and Open Laboratory Program of Ningbo University (No. Cxxkf 2008-067), Ningbo Natural Science Foundation (No. 2009A610134), Zhejiang Province Research Project (No. 2008C33020, and No. 2008F70052), Zhejiang Natural Sciences Foundation (No. Y207240 and No. Y207244), National Natural Science Foundation of China (No. 30872420), and K. C. Wong Magna Fund in Ningbo University.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, Y., Guo, J., Li, D. et al. Down-regulation of miR-31 expression in gastric cancer tissues and its clinical significance. Med Oncol 27, 685–689 (2010). https://doi.org/10.1007/s12032-009-9269-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12032-009-9269-x