Medical Oncology

, Volume 27, Issue 3, pp 618–623 | Cite as

Bone marrow hyaluronan and reticulin in patients with malignant disorders

  • G. SundströmEmail author
  • M. Hultdin
  • A. Engström-Laurent
  • I. M. S. Dahl
Original Paper


Myelofibrosis is commonly seen in patients with chronic myeloproliferative diseases and sometimes in myelodysplastic syndrome, acute leukaemia and lymphoproliferative diseases. The fibrotic process is evaluated by grading the amount of collagen deposited in the bone marrow interstitium. The established method to evaluate bone marrow fibrosis is staining for reticulin to visualise the collagen fibres. However, the extra cellular matrix does not only contain collagens but also other components, e.g. glycosaminoglycans of which hyaluronan is the most abundant. Hyaluronan is important for structural and cellular functions. Earlier studies have shown that there is a positive correlation between hyaluronan and reticulin staining in healthy volunteers and in patients with de novo acute myeloid leukaemia. In this study bone marrow biopsies from 43 patients with a malignant disease involving the bone marrow were compared with 18 patients with a malignant disease not involving the bone marrow. The intensity of hyaluronan grading was significantly higher in the patients with disease involving the bone marrow compared to the healthy controls but not compared to the patients without disease involving the bone marrow. The staining intensity of reticulin in the bone marrow was significantly higher in the patients with disease involving the bone marrow, compared to those without disease involving the bone marrow and to the controls. In all patients and the controls there was a correlation between hyaluronan and reticulin.


Hyaluronan Reticulin Fibrosis Bone marrow Tumour Malignant disorder 



This study was supported by grants from the Lion’s Cancer Research Foundation at Umeå University. We are indebted to Professor Anders Wahlin for critical examination, good statistical advice from Björn Tavelin, skilful technical assistance from Berith Lundström and excellent secretarial help from Kerstin Rosenqvist.


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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • G. Sundström
    • 1
    Email author
  • M. Hultdin
    • 2
  • A. Engström-Laurent
    • 3
  • I. M. S. Dahl
    • 4
  1. 1.Department of Oncology, Section of HaematologyNorrlands University HospitalUmeåSweden
  2. 2.Department of Medical Biosciences, PathologyUmeå UniversityUmeåSweden
  3. 3.Department of Public Health and Clinical MedicineUmeå UniversityUmeåSweden
  4. 4.Department of Medicine, Institute of Clinical MedicineUniversity of TromsöTromsöNorway

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