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Low molecular weight fraction secreted by SKOV3 cells expands peripheral CD4+CD25+ regulatory T cells and enhances their suppressive capacity

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Abstract

The increase of CD4+CD25+ regulatory T cells in patients with ovarian carcinoma has been verified. Here we investigated the effects of supernatant derived from ovarian carcinoma cell SKOV3 on peripheral regulatory T cells. Supernatant from SKOV3 was collected and fractionated into three different molecular weight fractions (MWFs). The proliferation of the CD4+CD25+ regulatory T cells cultured in complete RPMI 1640 medium with the different stimulators was detected. The phenotype (GITR and CTLA-4) of natural and expanded CD4+CD25+ T cells was detected by flow cytometry. Foxp3 mRNA expression of low MWF-expanded CD4+CD25+ T cells was detected by RT-PCR. Those expanded CD4+CD25+ regulatory T cells showed enhanced capacity to suppress CD4+CD25− T proliferation and increased expression of GITR and CTLA-4. In brief, low molecular weight fraction of supernatant secreted by SKOV3 could expand peripheral CD4+CD25+ regulatory T cells and enhance their suppressive function.

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Acknowledgments

Financial support for this work was provided by the project of Natural Scientific Foundation of Zhejiang Province (Grant code: Y207054) and the project of Zhejiang education office (20070245).

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Authors and Affiliations

  1. Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Xiao Li, Xiaoyun Wan, Yuyan Mao, Weiguo Lu & Xing Xie

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  1. Xiao Li
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  2. Xiaoyun Wan
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  3. Yuyan Mao
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  4. Weiguo Lu
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  5. Xing Xie
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Correspondence to Xing Xie.

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Li, X., Wan, X., Mao, Y. et al. Low molecular weight fraction secreted by SKOV3 cells expands peripheral CD4+CD25+ regulatory T cells and enhances their suppressive capacity. Med Oncol 27, 600–606 (2010). https://doi.org/10.1007/s12032-009-9255-3

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  • DOI: https://doi.org/10.1007/s12032-009-9255-3

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