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Medical Oncology

, Volume 27, Issue 2, pp 219–223 | Cite as

Immunohistochemical detection of the Raf kinase inhibitor protein in nonneoplastic gastric tissue and gastric cancer tissue

  • Jin Wang
  • Yong-Hong Yang
  • An-Qun Wang
  • Bing Yao
  • Gang Xie
  • Gang Feng
  • Yu Zhang
  • Zong-Shu Cheng
  • Li Hui
  • Tang-Zhi Dai
  • Xiao-Bo DuEmail author
  • Dong WangEmail author
Original Paper

Abstract

Expression of the Raf kinase inhibitor protein (RKIP), a metastasis suppressor, is high in normal tissues, low in primary cancers, and lowest or absent in metastatic cancers. Here, we studied the expression of RKIP in nonneoplastic gastric tissue and gastric cancer tissue by immunohistochemistry (IHC) and evaluated its role in the genesis and metastasis of gastric cancer. RKIP immunoreactivity was evaluated in 40 samples of nonneoplastic gastric tissues and 75 samples of gastric cancer tissues. Among the 40 samples of nonneoplastic gastric tissue, 35 (87.5%) were positive for RKIP expression and 5 (12.5%) were negative; in the 75 samples of primary gastric cancer tissue, 39 (52%) were positive for RKIP expression and 36 (48%) were negative. Among 26 samples of metastatic lymph node tissues, 5 (19.2%) were positive for RKIP expression and 21 (80.8%) were negative. RKIP expression level was highest in nonneoplastic gastric tissue, low in primary gastric cancer tissue, and lowest or undetectable in metastatic gastric cancer tissue. Our data suggest that RKIP may play a role in the genesis and metastasis of gastric cancer.

Keywords

Immunohistochemical Raf kinase inhibitor protein Nonneoplastic gastric tissue Gastric cancer tissue 

Notes

Acknowledgements

This work was supported by Scientific Research Projects of Sichuan Provincial Health Department (No:080216).

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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Jin Wang
    • 1
    • 2
  • Yong-Hong Yang
    • 3
  • An-Qun Wang
    • 3
  • Bing Yao
    • 2
  • Gang Xie
    • 3
  • Gang Feng
    • 3
  • Yu Zhang
    • 2
  • Zong-Shu Cheng
    • 3
  • Li Hui
    • 2
  • Tang-Zhi Dai
    • 2
  • Xiao-Bo Du
    • 2
    • 4
    Email author
  • Dong Wang
    • 1
    • 4
    Email author
  1. 1.Department of SurgeryLuZhou Medical CollegeLuZhouPeople’s Republic of China
  2. 2.Department of OncologyMianYang Central HospitalMianYangPeople’s Republic of China
  3. 3.Department of PathologyMianYang Central HospitalMianYangPeople’s Republic of China
  4. 4.Department of SurgeryMianYang Central HospitalMianYangPeople’s Republic of China

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