Expression of the Raf kinase inhibitor protein (RKIP), a metastasis suppressor, is high in normal tissues, low in primary cancers, and lowest or absent in metastatic cancers. Here, we studied the expression of RKIP in nonneoplastic gastric tissue and gastric cancer tissue by immunohistochemistry (IHC) and evaluated its role in the genesis and metastasis of gastric cancer. RKIP immunoreactivity was evaluated in 40 samples of nonneoplastic gastric tissues and 75 samples of gastric cancer tissues. Among the 40 samples of nonneoplastic gastric tissue, 35 (87.5%) were positive for RKIP expression and 5 (12.5%) were negative; in the 75 samples of primary gastric cancer tissue, 39 (52%) were positive for RKIP expression and 36 (48%) were negative. Among 26 samples of metastatic lymph node tissues, 5 (19.2%) were positive for RKIP expression and 21 (80.8%) were negative. RKIP expression level was highest in nonneoplastic gastric tissue, low in primary gastric cancer tissue, and lowest or undetectable in metastatic gastric cancer tissue. Our data suggest that RKIP may play a role in the genesis and metastasis of gastric cancer.
Immunohistochemical Raf kinase inhibitor protein Nonneoplastic gastric tissue Gastric cancer tissue
This is a preview of subscription content, log in to check access.
This work was supported by Scientific Research Projects of Sichuan Provincial Health Department (No:080216).
Bernier I, Jolles P. Purification and characterization of a basic 23 kD acytosolic protein from bovine brain. Biochim Biophys Acta. 1984;790:174–81.PubMedGoogle Scholar
Fu Z, et al. Effects of raf kinase inhibitor protein expression on suppression of prostate cancer metastasis. J Natl Cancer Inst. 2003;95(12):878–89.PubMedCrossRefGoogle Scholar
Simister PC, Banfield MJ, Brady RL. The crystal structure of PEBP-2, a homologue of the PEBP/RKIP family. Acta Crystallogr D Biol Crystallogr. 2002;58(Pt 6/Pt 2):1077–80.CrossRefPubMedGoogle Scholar
Chatterjee D, et al. Inverse association between Raf Kinase inhibitory protein and signal transducers and activators of transcription 3 expression in gastric adenocarcinoma patients implications for clinical outcome. Clin Cancer Res. 2008;14:2994–3001. doi:10.1158/1078-0432.CCR-07-4496.CrossRefPubMedGoogle Scholar