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Identification of RKIP as a differentially tyrosine-phosphorylated protein in nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissues by phosphoproteomic approach

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Abstract

Aims To screen for differentially tyrosine-phosphorylated proteins between nasopharyngeal carcinoma (NPC) and normal nasopharyngeal epithelial tissues (NNET) to provide a basis for elucidate the molecular mechanisms of NPC carcinogenesis. Methods Two-dimensional (2-D) electrophoresis was applied to separate proteins from NPC and NNET, respectively, and 2-D Western blotting was performed to detect tyrosine-phosphorylated proteins using antiphosphotyrosine antibody. Differentially tyrosine-phosphorylated proteins were identified by electrospray ionization-quadrupole time-of-flight MS (ESI-Q-TOF MS). NetPhos software was used to predict the tyrosine-phosphorylation sites of the identified proteins, and Western blotting was used to detect the tyrosine-phosphorylated levels of RKIP in NPC and NNET. Results Twenty-five differentially tyrosine-phosphorylated proteins in the two types of tissues were found, 13 of which were identified by ESI-Q-TOF MS. Among the 13 identified proteins, tyrosine-phosphorylated levels of 7 proteins were increased, and those of 6 proteins were decreased in NPC compared with NNET. NetPhos software prediction showed that all the 13 identified proteins contained tyrosine phosphorylation sites, and the differentially tyrosine-phosphorylated level of RKIP in NPC and NNET was confirmed. Conclusion The 13 differentially tyrosine-phosphorylated proteins may be involved in the development and progression of NPC.

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Acknowledgments

This study was supported by a grant from National Key Basic Research Program of China (2001CB510207), Outstanding Scholars of New Era from Ministry of Education of China (2002-48), Lotus Scholars Program of Hunan Province, China (2007-362), and key research program from Science and Technology Committee of Hunan, China (04XK1001-1, 05SK1004-1, 06SK2004).

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Authors and Affiliations

  1. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, China

    Yan Chen, Can-E. Tang, Guo-Liang Ouyang, Lin Ruan, Mao-Yu Li, Peng-Fei Zhang, Cui Li, Hong Yi, Fang Peng, Jian-Ling Li, Zhu-Chu Chen & Zhi-Qiang Xiao

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  1. Yan Chen
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  2. Can-E. Tang
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  3. Guo-Liang Ouyang
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  4. Lin Ruan
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  5. Mao-Yu Li
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  6. Peng-Fei Zhang
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  7. Cui Li
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  8. Hong Yi
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  9. Fang Peng
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  10. Jian-Ling Li
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  11. Zhu-Chu Chen
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  12. Zhi-Qiang Xiao
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Corresponding author

Correspondence to Zhi-Qiang Xiao.

Additional information

Y. Chen and C.-E. Tang contributed equally to this study.

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Chen, Y., Tang, CE., Ouyang, GL. et al. Identification of RKIP as a differentially tyrosine-phosphorylated protein in nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissues by phosphoproteomic approach. Med Oncol 26, 463–470 (2009). https://doi.org/10.1007/s12032-008-9147-y

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  • DOI: https://doi.org/10.1007/s12032-008-9147-y

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