Abstract
This study explored the differences in glycogen synthase kinase-3 beta (GSK3β) gene polymorphisms between patients with schizophrenia and healthy controls and investigated the association between gene polymorphisms and plasma concentration of aripiprazole. We enrolled 127 patients with schizophrenia and 125 healthy controls from southern Fujian. The genotypes of the rs6438552, rs12630592, and rs3732361 loci of GSK3β were evaluated by sequencing with amplified polymerase chain reaction, and the plasma concentration of aripiprazole was determined by high-performance liquid chromatography-tandem mass spectrometry. All three loci of GSK3β had three genotypes each. The genotype distribution in each locus was not significantly different, but there was a significant difference in the allele frequency between the schizophrenia and control groups within each locus. Linkage disequilibrium analyses of the three single-nucleotide polymorphisms (SNPs) revealed strong linkage. The haplotype analysis results showed two haplotypes in the three SNPs of GSK3β. The plasma concentrations, dose-corrected concentrations, and normalized concentrations of aripiprazole were significantly different among the different genotypes of the three SNPs. In conclusion, the rs6438552, rs12630592, and rs3732361 loci of GSK3β may be involved in schizophrenia, and GSK3β gene polymorphism may be correlated with the plasma concentration of aripiprazole.
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Acknowledgements
We would like to thank Editage (www.editage.cn) for English language editing.
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This work was supported by the Medical Science and Technology Planning Project of Xiamen (Grant number 3502Z20194081) and National Nature Science Foundation of China (Grant number 81871058).
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Zhizhong Xu: Conceptualization, investigation, writing—original draft. Chunyan Wen: Formal analysis, writing—review and editing. Yinghua Huang: Methodology. Qianfa Yuan: Investigation. Xianhua Zhang: Investigation, methodology. Duoduo Lin: Resources. Liangsheng Liu: Resources. Wenqiang Wang: Conceptualization, project administration, funding acquisition.
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Xu, Z., Wen, C., Huang, Y. et al. Effects of Glycogen Synthase Kinase-3 Beta Gene Polymorphisms on the Plasma Concentration of Aripiprazole in Chinese Patients with Schizophrenia: A Preliminary Study. J Mol Neurosci 73, 76–83 (2023). https://doi.org/10.1007/s12031-022-02079-7
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DOI: https://doi.org/10.1007/s12031-022-02079-7