Abstract
Our previous study showed that the lncRNA UBE2R2-AS1 inhibits the growth and invasion of glioma cells and promotes apoptosis through the miR-877-3p/TLR4 pathway. In this study, it was further found that the expression of UBE2R2-AS1 in glioma tissues was decreased significantly, and gradually decreased with increasing clinical stage. Chi-square analysis showed that the expression of UBE2R2-AS1 was significantly correlated with the WHO stage of tumor and epilepsy. Using Kaplan–Meier univariate survival analysis, it was found that the expression of UBE2R2-AS1 correlated positively with the overall survival of patients with glioma, while multiple Cox regression analysis showed that the expression of UBE2R2-AS1 correlated positively with the overall survival of patients with glioma as a protective factor for glioma prognosis. The analysis of data from TCGA also showed that patients with high UBE2R2-AS1 levels or low miR-877-3p expression were more likely to have good survival outcomes. Further construction of a glioma xenograft model in nude mice showed that UBE2R2-AS1 overexpression inhibited the growth of tumors, and the inhibition of miR-877-3p expression had a similar effect. Simultaneous UBE2R2-AS1 overexpression and miR-877-3p inhibition further decreased the growth rate of tumors in nude mice. Taken together, the results of our study suggest that UBE2R2-AS1 is an important tumor suppressor gene in glioma, which may be a good marker and treatment target for the clinical detection of glioma.
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Data Availability
The data sets used and analyzed during the current study are available from the corresponding author on reasonable request.
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We thank Xiangya Hospital Central South University and Affiliated Hospital of Jiujiang University for hosting the research.
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This work was supported by Xiangya Hospital Central South University postdoctoral foundation.
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QL and YWP led the study design and prepared the manuscript; WX conducted most of the experiments; DDC and LC performed data analysis and interpretation; SQL performed the PCR analyses; JS collected and embedded the clinical specimens; JT prepared the reagents and chemicals.
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All patients involved in this study signed an informed consent in accordance with the Declaration of Helsinki, and the study was approved by the Ethics Committee of Xinqiao Hospital. All experiments were approved by the Animal Care Committee of Xinqiao Hospital of the Third Military Medical University and were performed in accordance with the National Institutes of Health Guidelines for the Treatment of Animals.
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Xu, W., Che, Dd., Chen, L. et al. UBE2R2-AS1 Inhibits Xenograft Growth in Nude Mice and Correlates with a Positive Prognosis in Glioma. J Mol Neurosci 71, 1605–1613 (2021). https://doi.org/10.1007/s12031-021-01793-y
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DOI: https://doi.org/10.1007/s12031-021-01793-y