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Regional Differences in S-Nitrosylation in the Cortex, Striatum, and Hippocampus of Juvenile Male Mice

Journal of Molecular Neuroscience volume 71, pages 2383–2392 (2021)Cite this article

Abstract

Nitric oxide (NO) is a multifunctional neurotransmitter that plays a major role in neuronal and synaptic functions. S-nitrosylation (SNO), the NO-mediated protein posttransitional modification (PTM), is known to regulate physiological and pathological processes in the brain. However, the physiological role in different neuroanatomical brain regions has not been well investigated. To understand the role of SNO in the brain of juvenile WT mice, we used SNOTRAP technology. We mapped the SNO-proteome in three different neuroanatomical regions: cortex, striatum, and hippocampus. By conducting systems biology analysis, we found that the three brain regions share similar biological processes (BP) including biogenesis and developmental processes. Exclusive and different BP and molecular functions were found for each of the regions. Unraveling the BP and signaling mechanisms of SNO in the cortex, striatum, and hippocampus may help to understand the functional differences between the three regions under physiological conditions.

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Data Availability

The data were deposited in ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier < PXD006907 > and < PXD010106 > .

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Acknowledgements

We acknowledge the Satell Family Foundation for their generous support.

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Authors and Affiliations

  1. Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

    Wajeha Hamoudi, Felix von Lendenfeld, Maryam Kartawy, Shira Mencer, Huda Suloh, Igor Khaliulin & Haitham Amal

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  1. Wajeha Hamoudi
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  2. Felix von Lendenfeld
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  3. Maryam Kartawy
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  4. Shira Mencer
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  5. Huda Suloh
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  6. Igor Khaliulin
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  7. Haitham Amal
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Contributions

W.H.: system biology (SB) analysis and writing the manuscript, F.VL.: SB analysis, M.K.: SB analysis and contribution to the discussion, S.M.: SB analysis, H.S.: SB analysis, I.K.: contributing to the discussion, H.A.: supervision the project and writing the manuscript.

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Correspondence to Haitham Amal.

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Hamoudi, W., von Lendenfeld, F., Kartawy, M. et al. Regional Differences in S-Nitrosylation in the Cortex, Striatum, and Hippocampus of Juvenile Male Mice. J Mol Neurosci 71, 2383–2392 (2021). https://doi.org/10.1007/s12031-021-01792-z

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  • DOI: https://doi.org/10.1007/s12031-021-01792-z

Keywords

  • Nitric oxide
  • S-nitrosylation
  • Cortex
  • Striatum
  • Hippocampus
  • System biology
  • Posttranslational modification
  • Proteomics
  • Brain