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Emerging role of miRNAs as liquid biopsy markers for prediction of glioblastoma multiforme prognosis

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Abstract

Serum miRNAs (miRs) have gained consideration as encouraging molecular markers for cancer diagnosis and prediction of prognosis. The authors aimed to identify the exact role of miR-17-5p, miR-125b, and miR-221 among glioblastoma multiforme (GBM) patients before and after standard treatment, and correlate their expression with survival pattern. The study included 25 GBM patients and 20 healthy controls. Serum miR-17-5p, miR-125b, and miR-221 expression were analyzed before and after treatment using quantitative real-time polymerase chain reaction (qPCR). The diagnostic efficacy for the tested miRs was evaluated using the receiver operating characteristic (ROC) curve, and the relation of miRs expression versus clinical criteria for GBM was assessed. Patients’ survival patterns were examined versus miRs expression levels. A significant difference was reported between miRs expression among the enrolled individuals. Both miR-17-5p and miR-221 reported significant elevations in GBM patients who: are above 60 years old, underwent biopsy resection, have a non-frontal lesion, with tumor size above 5 cm, and with performance status equals 2 according to the Eastern Cooperative Oncology Group (ECOG) Performance Status. With regard to miR-125b, a significant difference was detected according to surgery strategy, primary lesion of the tumor, and ECOG status. MiRs levels were significantly decreased for GBM patients after treatment. Survival patterns demonstrated an increase in miR-17-5p, miR-125b, and miR-221 in GBM patients with worse progression-free survival and among those with worse overall survival. Detection of serum miR-17-5p, miR-125b, and miR-221 aids in the prediction of prognosis and response to treatment strategy for GBM patients.

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Acknowledgments

The instruments listed in the current study were purchased through a grant from Science and Technology Development Fund (STDF) through Capacity Building Grant Fund (CBG) [No. 4940].

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Authors and Affiliations

  1. Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Dokki, Egypt

    Menha Swellam & Noha M. Bakr

  2. High Throughput Molecular and Genetic Laboratory, Centers of Excellence for Advanced Sciences, National Research Centre, Giza, Dokki, Egypt

    Menha Swellam & Noha M. Bakr

  3. Biochemistry Department, Faculty of Pharmacy, Translational Research Unit, Misr International University, Cairo, Egypt

    Hekmat M. El Magdoub

  4. Clinical Pharmacy Practice Department, Faculty of Pharmacy, The British University in Egypt, El-Sherouk City, Cairo, Egypt

    Marwa S. Hamza

  5. The Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt, El-Sherouk City, Cairo, Egypt

    Marwa S. Hamza

  6. Clinical Oncology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Lobna R. Ezz El Arab

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  1. Menha Swellam
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  2. Noha M. Bakr
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Contributions

Study conception and design: MS; NMB; HME; MSH and LRE. Provision of samples and clinical follow-up: LRE. Acquisition of data: NMB; HME; MSH and LRE. Analysis and interpretation of data: MS; NMB; HME and MSH. Drafting of manuscript: MS; NMB; HME and MSH. Critical revision: MS; NMB; HME; MSH and LRE.

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Correspondence to Hekmat M. El Magdoub.

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Swellam, M., Bakr, N.M., El Magdoub, H.M. et al. Emerging role of miRNAs as liquid biopsy markers for prediction of glioblastoma multiforme prognosis. J Mol Neurosci 71, 836–844 (2021). https://doi.org/10.1007/s12031-020-01706-5

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