Abstract
The proliferation and migration of vascular smooth muscle cells (VSMCs) are involved in the pathogenesis of intracranial aneurysm (IA) formation and rupture. Interleukin enhancer binding factor 2 (ILF2) is known as the nuclear factor of activated T cells and regulates cell growth. This study was aimed to explore the effects of ILF2 on IA progression. Human brain VSMCs (hBVSMCs) were transfected with pCDNA3.1(+), pCDNA3.1(+)-ILF2, siRNA-negative control, and siRNA-ILF2. The transfection efficiency was then evaluated by determining ILF2 expression. The cell viability and apoptosis were determined using Cell Counting Kit-8 and Annexin V-FITC cell apoptosis assay kit, respectively. Real-time quantification PCR (RT-qPCR) was applied to measure the expression levels of apoptosis-related and inflammation-related genes. Finally, western blot was used to detect the expression level of Fas cell surface death receptor 95 (CD95) and Caspase 8. Overexpression of ILF2 could significantly increase cell viability and decrease cell apoptosis (P < 0.05), while knock-down of ILF2 showed opposite trends for hBVSMCs on cell viability and apoptosis (P < 0.05). RT-qPCR results showed that ILF2 knock-down downregulated the expression levels of BCL2 apoptosis regulator (BCL2), transcriptional regulator Myc-like (c-Myc), and caspase 1 (ICE) whereas upregulated the expression levels of CD95, p21, p53, and interleukin-13 (IL-13). Additionally, the protein expression levels of CD95 and Caspase 8 were significantly decreased after ILF2 overexpression while were significantly increased after ILF2 knock-down (P < 0.05). ILF2 knock-down may inhibit cell viability and promote cell apoptosis of hBVSMCs by regulating the expression levels of apoptosis-related genes and suppressing inflammatory response.
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The dataset used and/or analyzed during the current study are available from the corresponding author on a reasonable request.
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This study was financially supported by the Pudong new area science and technology development fund (No. PKJ2018-Y04) and the Fundamental Research Funds for the Central Universities (No. 22120190215).
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Conceptualization: Chunlong Zhong, Zhiyang Sun, Cheng Yang, and Liang Wei; Methodology: Cheng Yang and Liang Wei; Formal analysis and investigation: Guangxue Wang, Keqin Li, Yanfei Zhang, and Hongxin Guan; Writing—original draft preparation: Liang Wei and Cheng Yang; Writing—review and editing: Chunlong Zhong and Zhiyang Sun; Supervision: Chunlong Zhong and Zhiyang Sun. All authors have read and approved the final version.
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Liang Wei and Cheng Yang are co-first authors
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Wei, L., Yang, C., Wang, G. et al. Interleukin Enhancer Binding Factor 2 Regulates Cell Viability and Apoptosis of Human Brain Vascular Smooth Muscle Cells. J Mol Neurosci 71, 225–233 (2021). https://doi.org/10.1007/s12031-020-01638-0
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DOI: https://doi.org/10.1007/s12031-020-01638-0