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Dynamic Changes of Beclin-1 in the Hippocampus of Male Mice with Vascular Dementia at Different Time Points

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Abstract

This study tried to investigate the dynamic changes of Beclin-1 in the hippocampus of male mice with vascular dementia (VD) at different time points. The mouse model of VD was established by the four-vessel blocking method. Then, the VD mice were randomly divided into five groups (n = 12) according to the disease duration: the 0.1-day model group, 0.5-day model group, 1-day model group, 3-day model group, 5-day model group and 14-day model group. In addition, all surgical procedures were the same in the sham group as those in the model groups, except the mice in the sham group were not subjected to clipping. The expression of Beclin-1, LC3B, p62, Bcl-2, Bax, BACE1, GFAP, MBP and ET-1 mRNA were determined by RT-PCR; the expression of Beclin-1 was detected by Western blot and immunofluorescence; the pathological characteristics of the hippocampus were observed by haematoxylin-eosin (HE) staining; and the correlation of Beclin-1 with other VD-related proteins was analysed by Pearson’s correlation. Compared with that in the sham group, the expression of Beclin-1, LC3B, Bax, BACE1, GFAP, MBP and ET-1 mRNA was increased in the VD mice at different time points (0.1 day, 0.5 day, 1 day, 3 days, 5 days and 14 days) (P < 0.05) and then remained relatively stable in the 0.5-day VD mice, whereas the p62 and Bcl-2 mRNA levels decreased (P < 0.05). Beclin-1 protein expression was significantly increased in the VD mice at different time points (P < 0.05). The hippocampus showed a certain degree of neuronal damage in the VD mice at different time points (P < 0.05). Additionally, certain correlations among LC3B, p62, Bcl-2, Bax, BACE1, GFAP, MBP, ET-1 and Beclin-1 were observed in this study. In conclusion, the results described above demonstrated that neuronal damage and dynamic stability of Beclin-1 expression were established in the VD male mice after 0.5 day by the four-vessel blocking method.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 81904104 and Grant No. 31900297) and the Administration of Traditional Chinese Medicine of Guangdong Province, China (Grant No. 20181114). The work was also supported by the Lingnan Normal University–level talent project (Grant No. ZL1801), the Natural Science Foundation of Guangdong Province of China (Grant No. 2018A030307037), the Hainan Natural Science Foundation of China (Grant No. 20168266), the Program of Hainan Association for Science and Technology Plans to Youth R&D Innovation (Grant No. HAST201635) and the Guangdong Provincial Key Laboratory of Research on Emergency in TCM (Grant No. 2017B030314176), and Research Grant of Guangdong Provincial Key Laboratory of Research on Emergency in TCM.

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Authors and Affiliations

  1. Department of Neurology, Guangdong Provincial Hospital of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, People’s Republic of China

    Minzhen Deng, Xiaoqin Zhong & Manting Huang

  2. School of Chemistry and Chemical Engineering, Lingnan Normal University, No. 29, Cunjin Road, Zhanjiang, 524048, People’s Republic of China

    Liping Huang

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  1. Minzhen Deng
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Correspondence to Liping Huang.

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All the animal experiments were approved by the Ethical Committee for Animal Care and Use of China State Institute of Pharmaceutical Industry.

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Deng, M., Huang, L., Zhong, X. et al. Dynamic Changes of Beclin-1 in the Hippocampus of Male Mice with Vascular Dementia at Different Time Points. J Mol Neurosci 70, 1611–1618 (2020). https://doi.org/10.1007/s12031-020-01591-y

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