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Phosphomimetic Mutation of Glycine Transporter GlyT1 C-Terminal PDZ Binding Motif Inhibits its Interactions with PSD95

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A Correction to this article was published on 09 January 2020

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ABSTRACT

Even though the abundance of GlyT1 in postsynaptic aspects of forebrain neurons is low, its previously reported interaction with postsynaptic density protein PSD95 represents a prototype of interaction, which might uncover the binding mechanism and regulation of the GlyT1 C-terminal PDZ motif. We used a phosphomimetic approach to mimic the potential phosphorylation of GlyT1 C-terminal serines 640, 643, 644, and 649 of the mouse GlyT1b subtype (mGlyT1b) (Uniprot P28571-2) and its effect on GlyT1-PSD95 PDZ interaction. Among them, only phosphomimetic mutation of serine 649 to aspartate, which resides in the minimal PDZ motif -SRI, significantly eliminated the interaction of the GlyT1 C-terminus with PSD95 PDZ domain 2. The effect was observed with recombinant fusion proteins as well as with GlyT1 and PSD95 expressed in tissue culture. Results indicate that phosphorylation of mouse GlyT1b serine 649 and equivalent serines of GlyT1a and GlyT1c subtypes might regulate the PDZ interaction of the GlyT1 C-terminal PDZ binding motif.

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Change history

  • 09 January 2020

    The original version of this article unfortunately contained two mistakes.

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Funding

This work was supported by VEGA Grant 2/0066/17.

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  1. Laboratory of Neurobiology, Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51, Bratislava, Slovakia

    Martina Baliova & Frantisek Jursky

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  1. Martina Baliova
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  2. Frantisek Jursky
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Correspondence to Martina Baliova or Frantisek Jursky.

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The original version of this article was revised: This article unfortunately contained two mistakes. In the html version, Figure 1 was missing and was replaced by Figure 2, resulting to identical version of both figures. While in the PDF version, Figures 1 and 2 were inadvertently interchanged.

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Baliova, M., Jursky, F. Phosphomimetic Mutation of Glycine Transporter GlyT1 C-Terminal PDZ Binding Motif Inhibits its Interactions with PSD95. J Mol Neurosci 70, 488–493 (2020). https://doi.org/10.1007/s12031-019-01435-4

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