Abstract
Sepsis-associated encephalopathy (SAE) is a common complication of sepsis, and has no generally accepted treatment due to its complicated pathophysiology. Previously, we demonstrated the protective role of neuroglobin (Ngb) in SAE rats, but the exact mechanism has not been determined. To investigate the potential neuroprotective roles and mechanisms of Ngb, Sprague-Dawley rats were used. Overexpression of Ngb via intracerebroventricular injection with Ngb plasmids attenuated brain damage assessed by hematoxylin and eosin (HE) staining and neurological dysfunction assessed by Morris water maze test. Western blot analysis also showed that the phosphorylation of Akt increased and the protein level of Bax decreased. Furthermore, the protective effect can be abolished by PI3K/Akt pathway inhibitor LY294002. Our results demonstrate that Ngb can protect rats from SAE via a PI3K/Akt/Bax-dependent mechanism.
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Abbreviations
- Ngb:
-
neuroglobin
- SAE:
-
Sepsis-associated encephalopathy
- CLP:
-
Cecal ligation and puncture
- PI3K:
-
Phosphatidylinositol-4,5-bisphosphate 3-kinase
- Akt:
-
Protein kinase B
- LY:
-
LY294002
- OE:
-
Overexpression
- NC:
-
Negative control
- MWM:
-
Morris water maze
- i.p:
-
Intraperitoneal
- HE:
-
Hematoxylin and eosin
- MOMP:
-
Mitochondrial outer membrane permeabilization
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (no. 81401099 and no. 81671960).
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Deng, S., Ai, Y., Gong, H. et al. Neuroglobin Protects Rats from Sepsis-Associated Encephalopathy via a PI3K/Akt/Bax-Dependent Mechanism. J Mol Neurosci 63, 1–8 (2017). https://doi.org/10.1007/s12031-017-0933-x
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DOI: https://doi.org/10.1007/s12031-017-0933-x